Fischer L, Thievessen I (2023)
Publication Type: Book chapter / Article in edited volumes
Publication year: 2023
Publisher: Humana Press Inc.
Series: Methods in Molecular Biology
Book Volume: 2644
Pages Range: 371-385
DOI: 10.1007/978-1-0716-3052-5_24
One of the most commonly assessed parameters in cellular analyses is the proliferative activity of a cell population. The fluorescence ubiquitin cell cycle indicator (FUCCI)-based system allows live and in vivo observation of cell cycle progression. Based on the mutually exclusive activity of two fluorescently labeled proteins cdt1 and geminin during the G0/1 and S/G2/M phases of the cell cycle, individual cells can be assigned to their respective cell cycle phase by fluorescence imaging of the nucleus. Here, we describe the generation of NIH/3T3 cells containing the FUCCI reporter system by lentiviral transduction and their use in 3D culture assays. The protocol can be adapted to other cell lines.
APA:
Fischer, L., & Thievessen, I. (2023). FUCCI Reporter Gene-Based Cell Cycle Analysis. In (pp. 371-385). Humana Press Inc..
MLA:
Fischer, Lena, and Ingo Thievessen. "FUCCI Reporter Gene-Based Cell Cycle Analysis." Humana Press Inc., 2023. 371-385.
BibTeX: Download