Schneidereit D, Nübler S, Friedrich O (2023)
Publication Type: Book chapter / Article in edited volumes
Publication year: 2023
Publisher: Humana Press Inc.
Edited Volumes: Cell Viability Assays (2nd Edt.)
Series: Methods in Molecular Biology
Book Volume: 2644
Pages Range: 267-285
DOI: 10.1007/978-1-0716-3052-5_17
The architectural structure of cells is essential for the cells’ function, which becomes especially apparent in the highly “structure functionally” tuned skeletal muscle cells. Here, structural changes in the microstructure can have a direct impact on performance parameters, such as isometric or tetanic force production. The microarchitecture of the actin-myosin lattice in muscle cells can be detected noninvasively in living cells and in 3D by using second harmonic generation (SHG) microscopy, forgoing the need to alter samples by introducing fluorescent probes into them. Here, we provide tools and step-by-step protocols to guide the processes of obtaining SHG microscopy image data from samples, as well as extracting characteristic values from the image data to quantify the cellular microarchitecture using characteristic patterns of myofibrillar lattice alignments.
APA:
Schneidereit, D., Nübler, S., & Friedrich, O. (2023). Second Harmonic Generation Morphometry of Muscle Cytoarchitecture in Living Cells. In Friedrich O, Gilbert DF (Eds.), Cell Viability Assays (2nd Edt.). (pp. 267-285). Humana Press Inc..
MLA:
Schneidereit, Dominik, Stefanie Nübler, and Oliver Friedrich. "Second Harmonic Generation Morphometry of Muscle Cytoarchitecture in Living Cells." Cell Viability Assays (2nd Edt.). Ed. Friedrich O, Gilbert DF, Humana Press Inc., 2023. 267-285.
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