Observing Membrane and Cell Adhesion via Reflection Interference Contrast Microscopy

Abdelrahman A, Smith AS, Sengupta K (2023)

Publication Type: Book chapter / Article in edited volumes

Publication year: 2023

Journal

Methods in Molecular Biology Humana Press

Publisher: Humana Press Inc.

Series: Methods in Molecular Biology

Book Volume: 2654

Pages Range: 123-135

DOI: 10.1007/978-1-0716-3135-5_8

Abstract

Reflection interference contrast microscopy (RICM) is an optical microscopy technique ideally suited for imaging adhesion. While RICM (and the closely related interference reflection microscopy (IRM)) has been extensively used qualitatively or semiquantitatively to image cells, including immune cells, it can also be used quantitatively to measure membrane to surface distance, especially for model membranes. Here, we present a protocol for RICM and IRM imaging and the details of semiquantitative and quantitative analysis.

Authors with CRIS profile

Ana-Suncana Smith Physics Underlying Life Science

Involved external institutions

Aix-Marseille University / Aix-Marseille Université FR France (FR)

How to cite

APA:

Abdelrahman, A., Smith, A.-S., & Sengupta, K. (2023). Observing Membrane and Cell Adhesion via Reflection Interference Contrast Microscopy. In (pp. 123-135). Humana Press Inc..

MLA:

Abdelrahman, Ahmed, Ana-Suncana Smith, and Kheya Sengupta. "Observing Membrane and Cell Adhesion via Reflection Interference Contrast Microscopy." Humana Press Inc., 2023. 123-135.

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