Fast detection of SARS-CoV2 Antibodies by the use of critical off-set magnetic particle spectroscopy (COMPASS)

Friedrich B, Tietze R, Rückert MA, Kampf T, Hennig T, Dölken L, Behr VC, Alexiou C, Vogel P, Lyer S (2023)

Publication Type: Journal article

Publication year: 2023

Journal

International Journal of Magnetic Particle Imaging Infinite Science Publishing

Book Volume: 9

Article Number: 2303063

DOI: 10.18416/IJMPI.2023.2303063

Abstract

Current bioassays for detection of antibodies or antigens such as ELISA (Enzyme-linked Immunosorbent Assay) are relatively inflexible, expensive and time-consuming. Upcoming methods, such as ACS (AC susceptometry) or MPS (Magnetic Particle Spectroscopy), exploit the magnetization response of functionalized magnetic nanoparticles (MNP) ensembles to assess specific information about the MNP mobility as well as conjugations of chemical or biological compounds on their surface. Both methods have shown promising results in the past but cannot reach the sensitivity of above-mentioned techniques. We used a novel method based on a modified MPS being sensitive to minimal changes in mobility of MNP ensembles. This facilitates robust and easy-to-handle measurements of minimal changes in the diameter of MNPs. As an example, we detected SARS-CoV-2 antibodies binding to the S1 antigen on the surface of functionalized MNPs. Without any purification or incubation, we could show a sensitivity of less than 50 ng/mL of SARS-CoV-2 antibodies in samples.

Authors with CRIS profile

Bernhard Friedrich Department of Otorhinolaryngology – Head and Neck Surgery (HNO-Klinik) Rainer Tietze Department of Otorhinolaryngology – Head and Neck Surgery (HNO-Klinik) Christoph Alexiou Medizinische Fakultät Stefan Lyer Department of Otorhinolaryngology – Head and Neck Surgery (HNO-Klinik)

Involved external institutions

Julius-Maximilians-Universität Würzburg DE Germany (DE) Helmholtz-Institut für RNA-basierte Infektionsforschung (HIRI) DE Germany (DE)

How to cite

APA:

Friedrich, B., Tietze, R., Rückert, M.A., Kampf, T., Hennig, T., Dölken, L.,... Lyer, S. (2023). Fast detection of SARS-CoV2 Antibodies by the use of critical off-set magnetic particle spectroscopy (COMPASS). International Journal of Magnetic Particle Imaging, 9. https://doi.org/10.18416/IJMPI.2023.2303063

MLA:

Friedrich, Bernhard, et al. "Fast detection of SARS-CoV2 Antibodies by the use of critical off-set magnetic particle spectroscopy (COMPASS)." International Journal of Magnetic Particle Imaging 9 (2023).

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