A Novel Window into Angiogenesis — Intravital Microscopy in the AV-Loop-Model

Vaghela R, Arkudas A, Gage D, Körner C, von Hörsten S, Salehi S, Horch RE, Hessenauer M (2023)

Publication Type: Journal article

Publication year: 2023

Journal

Cells MDPI

Book Volume: 12

Article Number: 261

Journal Issue: 2

DOI: 10.3390/cells12020261

Abstract

Due to the limitations of current in vivo experimental designs, our comprehensive knowledge of vascular development and its implications for the development of large-scale engineered tissue constructs is very limited. Therefore, the purpose of this study was to develop unique in vivo imaging chambers that allow the live visualization of cellular processes in the arteriovenous (AV) loop model in rats. We have developed two different types of chambers. Chamber A is installed in the skin using the purse sting fixing method, while chamber B is installed subcutaneously under the skin. Both chambers are filled with modified gelatin hydrogel as a matrix. Intravital microscopy (IVM) was performed after the injection of fluorescein isothiocyanate (FITC)-labeled dextran and rhodamine 6G dye. The AV loop was functional for two weeks in chamber A and allowed visualization of the leukocyte trafficking. In chamber B, microvascular development in the AV loop could be examined for 21 days. Quantification of the microvascular outgrowth was performed using Fiji-ImageJ. Overall, by combining these two IVM chambers, we can comprehensively understand vascular development in the AV loop tissue engineering model¯.

Authors with CRIS profile

Ravikumar Vaghela Department of Plastic and Hand Surgery Andreas Arkudas Medizinische Fakultät Daniel Gage Lehrstuhl für Werkstoffwissenschaften (Werkstoffkunde und Technologie der Metalle) Carolin Körner Lehrstuhl für Werkstoffwissenschaften (Werkstoffkunde und Technologie der Metalle) Stephan von Hörsten Professur für Experimentelle Biomedizin Raymund Horch Professur für Plastische Chirurgie und Handchirurgie

Involved external institutions

Universität Bayreuth DE Germany (DE)

How to cite

APA:

Vaghela, R., Arkudas, A., Gage, D., Körner, C., von Hörsten, S., Salehi, S.,... Hessenauer, M. (2023). A Novel Window into Angiogenesis — Intravital Microscopy in the AV-Loop-Model. Cells, 12(2). https://dx.doi.org/10.3390/cells12020261

MLA:

Vaghela, Ravikumar, et al. "A Novel Window into Angiogenesis — Intravital Microscopy in the AV-Loop-Model." Cells 12.2 (2023).

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