3D-printed β-TCP/S53P4 bioactive glass scaffolds coated with tea tree oil: Coating optimization, in vitro bioactivity and antibacterial properties

Alves APN, Arango Ospina M, Oliveira RLMS, Ferreira IM, de Moraes EG, Hartmann M, de Oliveira APN, Boccaccini AR, de Sousa Trichês E (2022)

Publication Type: Journal article

Publication year: 2022

Journal

Journal of Biomedical Materials Research Part B: Applied Biomaterials Wiley

DOI: 10.1002/jbm.b.35198

Abstract

Bone infection treatment is a significant challenge for the orthopedic field. 3D printing is a promising technology to produce scaffolds with customized architecture, able to stimulate and support bone growth. β-TCP and S53P4 bioactive glass (BG) are well-known biomaterials for scaffold manufacturing. However, a multifunctional scaffold, able to inhibit microbial proliferation at the defect site, is of increasing interest to avoid infection recurrence. Tea tree oil (TTO) has aroused interest as an antimicrobial agent to minimize the use of antibiotics. Therefore, combining the regenerative potential of a bioceramic with TTO's antimicrobial properties could result in a scaffold capable of stimulating tissue growth and treating infections. In this context, this study aimed to produce and characterize 3D-printed β-TCP/S53P4 BG scaffolds coated with TTO. Scaffolds morphological and chemical characterizations were carried out through XDR, SEM, and FTIR analysis. β-TCP/S53P4 BG scaffolds showed a compressive strength of ~2 MPa and 53 ± 2% of porosity. The scaffolds were coated by two different procedures, using an ethanol/TTO (EtOH/TTO) and a gelatin/TTO (Gel/TTO) solution with 5, 10, and 15% (v/v) TTO. The addition of TTO decreased MG-63 cell viability for both coating groups, but the Gel/TTO group showed higher cell viability. The antibacterial activity of the coated scaffolds was evaluated against S. aureus and higher inhibition of colony formation was found for Gel/TTO group. Therefore, the coating with Gel/TTO was effective in terms of antibacterial activity and cell viability. Such Gel/TTO coated β-TCP/S53P4 BG scaffolds are proposed for antibacterial bone tissue engineering.

Authors with CRIS profile

Marcela Arango Ospina Lehrstuhl für Werkstoffwissenschaften (Biomaterialien) Malte Hartmann Lehrstuhl für Werkstoffwissenschaften (Biomaterialien) Aldo Boccaccini Lehrstuhl für Werkstoffwissenschaften (Biomaterialien)

Involved external institutions

Federal University of São Paulo / Universidade Federal de São Paulo (UNIFESP) BR Brazil (BR) Federal University of Santa Catarina / Universidade Federal de Santa Catarina (UFSC) BR Brazil (BR)

How to cite

APA:

Alves, A.P.N., Arango Ospina, M., Oliveira, R.L.M.S., Ferreira, I.M., de Moraes, E.G., Hartmann, M.,... de Sousa Trichês, E. (2022). 3D-printed β-TCP/S53P4 bioactive glass scaffolds coated with tea tree oil: Coating optimization, in vitro bioactivity and antibacterial properties. Journal of Biomedical Materials Research Part B: Applied Biomaterials. https://dx.doi.org/10.1002/jbm.b.35198

MLA:

Alves, Ana Paula Nogueira, et al. "3D-printed β-TCP/S53P4 bioactive glass scaffolds coated with tea tree oil: Coating optimization, in vitro bioactivity and antibacterial properties." Journal of Biomedical Materials Research Part B: Applied Biomaterials (2022).

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