Critical Offset Magnetic PArticle SpectroScopy for rapid and highly sensitive medical point-of-care diagnostics

Vogel P, Rückert MA, Friedrich B, Tietze R, Lyer S, Kampf T, Hennig T, Dölken L, Alexiou C, Behr VC (2022)

Publication Type: Journal article

Publication year: 2022

Journal

Nature Communications Nature Publishing Group: Nature Communications

Book Volume: 13

Article Number: 7230

Journal Issue: 1

DOI: 10.1038/s41467-022-34941-y

Abstract

Magnetic nanoparticles (MNPs) have been adapted for many applications, e.g., bioassays for the detection of biomarkers such as antibodies, by controlled engineering of specific surface properties. Specific measurement of such binding states is of high interest but currently limited to highly sensitive techniques such as ELISA or flow cytometry, which are relatively inflexible, difficult to handle, expensive and time-consuming. Here we report a method named COMPASS (Critical-Offset-Magnetic-Particle-SpectroScopy), which is based on a critical offset magnetic field, enabling sensitive detection to minimal changes in mobility of MNP ensembles, e.g., resulting from SARS-CoV-2 antibodies binding to the S antigen on the surface of functionalized MNPs. With a sensitivity of 0.33 fmole/50 µl (≙7 pM) for SARS-CoV-2-S1 antibodies, measured with a low-cost portable COMPASS device, the proposed technique is competitive with respect to sensitivity while providing flexibility, robustness, and a measurement time of seconds per sample. In addition, initial results with blood serum demonstrate high specificity.

Authors with CRIS profile

Bernhard Friedrich Department of Otorhinolaryngology – Head and Neck Surgery (HNO-Klinik) Rainer Tietze Department of Otorhinolaryngology – Head and Neck Surgery (HNO-Klinik) Stefan Lyer Department of Otorhinolaryngology – Head and Neck Surgery (HNO-Klinik) Christoph Alexiou Medizinische Fakultät

Involved external institutions

Julius-Maximilians-Universität Würzburg DE Germany (DE)

How to cite

APA:

Vogel, P., Rückert, M.A., Friedrich, B., Tietze, R., Lyer, S., Kampf, T.,... Behr, V.C. (2022). Critical Offset Magnetic PArticle SpectroScopy for rapid and highly sensitive medical point-of-care diagnostics. Nature Communications, 13(1). https://doi.org/10.1038/s41467-022-34941-y

MLA:

Vogel, Patrick, et al. "Critical Offset Magnetic PArticle SpectroScopy for rapid and highly sensitive medical point-of-care diagnostics." Nature Communications 13.1 (2022).

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