Vascularization of Poly-ε-Caprolactone-Collagen I-Nanofibers with or without Sacrificial Fibers in the Neurotized Arteriovenous Loop Model

Kratzer S, Arkudas A, Himmler M, Schubert DW, Schneidereit D, Bauer J, Friedrich O, Horch RE, Cai A (2022)

Publication Type: Journal article, Original article

Publication year: 2022

Journal

Cells MDPI

Book Volume: 11

Pages Range: 3774

Issue: 23

DOI: 10.3390/cells11233774

Abstract

Electrospun nanofibers represent an ideal matrix for the purpose of skeletal muscle tissue engineering due to their highly aligned structure in the nanoscale, mimicking the extracellular matrix of skeletal muscle. However, they often consist of high-density packed fibers, which might impair vascularization. The integration of polyethylene oxide (PEO) sacrificial fibers, which dissolve in water, enables the creation of less dense structures. This study examines potential benefits of poly-ε-caprolactone-collagen I-PEO-nanoscaffolds (PCP) in terms of neovascularization and distribution of newly formed vessels compared to poly-ε-caprolactone -collagen I-nanoscaffolds (PC) in a modified arteriovenous loop model in the rat. For this purpose, the superficial inferior epigastric artery and vein as well as a motor nerve branch were integrated into a multilayer three-dimensional nanofiber scaffold construct, which was enclosed by an isolation chamber. Numbers and spatial distribution of sprouting vessels as well as macrophages were analyzed via immunohistochemistry after two and four weeks of implantation. After four weeks, aligned PC showed a higher number of newly formed vessels, regardless of the compartments formed in PCP by the removal of sacrificial fibers. Both groups showed cell influx and no difference in macrophage invasion. In this study, a model of combined axial vascularization and neurotization of a PCL-collagen I-nanofiber construct could be established for the first time. These results provide a foundation for the in vivo implantation of cells, taking a major step towards the generation of functional skeletal muscle tissue.

Authors with CRIS profile

Simon Kratzer Medizinische Fakultät Andreas Arkudas Medizinische Fakultät Marcus Himmler Lehrstuhl für Werkstoffwissenschaften (Polymerwerkstoffe) Dirk Schubert Lehrstuhl für Werkstoffwissenschaften (Polymerwerkstoffe) Dominik Schneidereit Lehrstuhl für Medizinische Biotechnologie Julian Bauer Lehrstuhl für Medizinische Biotechnologie Oliver Friedrich Lehrstuhl für Medizinische Biotechnologie Raymund Horch Professur für Plastische Chirurgie und Handchirurgie Aijia Cai Department of Plastic and Hand Surgery

How to cite

APA:

Kratzer, S., Arkudas, A., Himmler, M., Schubert, D.W., Schneidereit, D., Bauer, J.,... Cai, A. (2022). Vascularization of Poly-ε-Caprolactone-Collagen I-Nanofibers with or without Sacrificial Fibers in the Neurotized Arteriovenous Loop Model. Cells, 11, 3774. https://doi.org/10.3390/cells11233774

MLA:

Kratzer, Simon, et al. "Vascularization of Poly-ε-Caprolactone-Collagen I-Nanofibers with or without Sacrificial Fibers in the Neurotized Arteriovenous Loop Model." Cells 11 (2022): 3774.

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