Long-term stimulation with alternating electric fields modulates the differentiation and mineralization of human pre-osteoblasts

Sahm F, Freiin Grote V, Zimmermann J, Haack F, Uhrmacher AM, van Rienen U, Bader R, Detsch R, Jonitz-Heincke A (2022)

Publication Type: Journal article

Publication year: 2022

Journal

Frontiers in Physiology Frontiers Media

Book Volume: 13

Article Number: 965181

DOI: 10.3389/fphys.2022.965181

Abstract

Biophysical stimulation by electric fields can promote bone formation in bone defects of critical size. Even though, long-term effects of alternating electric fields on the differentiation of osteoblasts are not fully understood. Human pre-osteoblasts were stimulated over 31 days to gain more information about these cellular processes. An alternating electric field with 0.7 Vrms and 20 Hz at two distances was applied and viability, mineralization, gene expression, and protein release of differentiation factors were analyzed. The viability was enhanced during the first days of stimulation. A higher electric field resulted in upregulation of typical osteogenic markers like osteoprotegerin, osteopontin, and interleukin-6, but no significant changes in mineralization. Upregulation of the osteogenic markers could be detected with a lower electric field after the first days of stimulation. As a significant increase in the mineralized matrix was identified, an enhanced osteogenesis due to low alternating electric fields can be assumed.

Authors with CRIS profile

Rainer Detsch Lehrstuhl für Werkstoffwissenschaften (Biomaterialien)

Involved external institutions

Universitätsmedizin Rostock DE Germany (DE) Universität Rostock DE Germany (DE)

How to cite

APA:

Sahm, F., Freiin Grote, V., Zimmermann, J., Haack, F., Uhrmacher, A.M., van Rienen, U.,... Jonitz-Heincke, A. (2022). Long-term stimulation with alternating electric fields modulates the differentiation and mineralization of human pre-osteoblasts. Frontiers in Physiology, 13. https://dx.doi.org/10.3389/fphys.2022.965181

MLA:

Sahm, Franziska, et al. "Long-term stimulation with alternating electric fields modulates the differentiation and mineralization of human pre-osteoblasts." Frontiers in Physiology 13 (2022).

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