Label-Free Characterization and Quantification of Mucosal Inflammation in Common Murine Colitis Models With Multiphoton Imaging

Kreiß L, Thoma OM, Lemire S, Lechner K, Carlé BE, Dilipkumar A, Kunert T, Scheibe K, Heichler C, Merten AL, Weigmann B, Neufert C, Hildner K, Vieth M, Neurath M, Friedrich O, Schürmann S, Waldner M (2022)

Publication Type: Journal article, Original article

Publication year: 2022

Journal

Inflammatory Bowel Diseases Wiley

DOI: 10.1093/ibd/izac114

Abstract

Background Clinical challenges in inflammatory bowel diseases require microscopic in vivo evaluation of inflammation. Here, label-free imaging holds great potential, and recently, our group demonstrated the advantage of using in vivo multiphoton endomicroscopy for longitudinal animal studies. This article extends our previous work by in-depth analysis of label-free tissue features in common colitis models quantified by the multiphoton colitis score (MCS). Methods Fresh mucosal tissues were evaluated from acute and chronic dextran sulfate sodium (DSS), TNBS, oxazolone, and transfer colitis. Label-free imaging was performed by using second harmonic generation and natural autofluorescence. Morphological changes in mucosal crypts, collagen fibers, and cellularity in the stroma were analyzed and graded. Results Our approach discriminated between healthy (mean MCS = 2.5) and inflamed tissue (mean MCS > 5) in all models, and the MCS was validated by hematoxylin and eosin scoring of the same samples (85.2% agreement). Moreover, specific characteristics of each phenotype were identified. While TNBS, oxazolone, and transfer colitis showed high cellularity in stroma, epithelial damage seemed specific for chronic, acute DSS and transfer colitis. Crypt deformations were mostly observed in acute DSS. Conclusions Quantification of label-free imaging is promising for in vivo endoscopy. In the future, this could be valuable for monitoring of inflammatory pathways in murine models, which is highly relevant for the development of new inflammatory bowel disease therapeutics.

Authors with CRIS profile

Lucas Kreiß Department of Medicine 1 – Gastroenterology, Pneumology and Endocrinology Oana-Maria Thoma Department of Medicine 1 – Gastroenterology, Pneumology and Endocrinology Sarah Lemire Professur für Funktionelle Bildgebung in der Medizin Birgitta-Elisabeth Carlé Lehrstuhl für Medizinische Biotechnologie (MBT) Ashwathama Dilipkumar Lehrstuhl für Medizinische Biotechnologie (MBT) Timo Kunert Department of Medicine 1 – Gastroenterology, Pneumology and Endocrinology Kristina Koop Department of Medicine 1 – Gastroenterology, Pneumology and Endocrinology Anna-Lena Merten Lehrstuhl für Medizinische Biotechnologie (MBT) Benno Weigmann Medizinische Fakultät Clemens Neufert Medizinische Fakultät Kai Hildner Juniorprofessur für Pneumologie/ Immunologie Michael Vieth
Markus Neurath Lehrstuhl für Innere Medizin I (Medizin 1) Oliver Friedrich Lehrstuhl für Medizinische Biotechnologie (MBT) Sebastian Schürmann Lehrstuhl für Medizinische Biotechnologie (MBT) Maximilian Waldner Lehrstuhl für Innere Medizin I (Medizin 1)

Additional Organisation(s)

Interdisziplinäres Zentrum für Klinische Forschung (IZKF)

Related research project(s)

J085: Analysis of cell-type-specific roles and crosstalk of IL36R signaling in gut inflammation and fibrosis Nov. 16, 2020 - Nov. 15, 2023

Involved external institutions

Klinikum Bayreuth DE Germany (DE)

How to cite

APA:

Kreiß, L., Thoma, O.-M., Lemire, S., Lechner, K., Carlé, B.-E., Dilipkumar, A.,... Waldner, M. (2022). Label-Free Characterization and Quantification of Mucosal Inflammation in Common Murine Colitis Models With Multiphoton Imaging. Inflammatory Bowel Diseases. https://doi.org/10.1093/ibd/izac114

MLA:

Kreiß, Lucas, et al. "Label-Free Characterization and Quantification of Mucosal Inflammation in Common Murine Colitis Models With Multiphoton Imaging." Inflammatory Bowel Diseases (2022).

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