Improved method for efficient generation of functional neurons from murine neural progenitor cells

Soni A, Kluetsch D, Hu X, Houtman J, Rund N, Mccloskey A, Mertens J, Schafer ST, Amin H, Toda T (2021)

Publication Type: Journal article

Publication year: 2021

Journal

Cells MDPI

Book Volume: 10

Article Number: 1894

Journal Issue: 8

DOI: 10.3390/cells10081894

Abstract

Neuronal culture was used to investigate neuronal function in physiological and pathological conditions. Despite its inevitability, primary neuronal culture remained a gold standard method that requires laborious preparation, intensive training, and animal resources. To circum-vent the shortfalls of primary neuronal preparations and efficiently give rise to functional neurons, we combine a neural stem cell culture method with a direct cell type-conversion approach. The lucidity of this method enables the efficient preparation of functional neurons from mouse neural progenitor cells on demand. We demonstrate that induced neurons (NPC-iNs) by this method make synaptic connections, elicit neuronal activity-dependent cellular responses, and develop functional neuronal networks. This method will provide a concise platform for functional neuronal assess-ments. This indeed offers a perspective for using these characterized neuronal networks for investigating plasticity mechanisms, drug screening assays, and probing the molecular and biophysical basis of neurodevelopmental and neurodegenerative diseases.

Authors with CRIS profile

Tomohisa Toda

Involved external institutions

Salk Institute for Biological Studies US United States (USA) (US) Deutsches Zentrum für Neurodegenerative Erkrankungen (DZNE) DE Germany (DE) Leopold-Franzens-Universität Innsbruck / University of Innsbruck AT Austria (AT)

How to cite

APA:

Soni, A., Kluetsch, D., Hu, X., Houtman, J., Rund, N., Mccloskey, A.,... Toda, T. (2021). Improved method for efficient generation of functional neurons from murine neural progenitor cells. Cells, 10(8). https://dx.doi.org/10.3390/cells10081894

MLA:

Soni, Abhinav, et al. "Improved method for efficient generation of functional neurons from murine neural progenitor cells." Cells 10.8 (2021).

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