Size exclusion chromatography (SEC)

Peukert W, Kaspereit M, Hofe T, Gromotka L (2022)

Publication Type: Book chapter / Article in edited volumes

Publication year: 2022

Edited Volumes: Particle Separation Techniques

Pages Range: 409-447

DOI: 10.1016/B978-0-323-85486-3.00003-2

Abstract

Size exclusion chromatography (SEC) is a liquid phase chromatographic technique for separating molecules, proteins, macromolecules, and nanoparticles (below 100 nm) solely according to their hydrodynamic volume in solution. Classification depends on the size- and shape-dependent exclusion of components in the pores of the stationary phase material, that is, larger and less mobile components leave the column first. Ideally, the separation is purely driven by entropy, the absence of strong enthalpic interactions encompasses mild operating conditions. SEC is a standard method in polymer science and technology for the characterization of molecular weight distributions. SEC is also well established for preparative applications, for example, for the separation of proteins from aggregates in biotechnology. The size-dependent classification of nanoparticles is a promising new field of application.

Authors with CRIS profile

Wolfgang Peukert Lehrstuhl für Feststoff- und Grenzflächenverfahrenstechnik Malte Kaspereit Professur für Thermische Verfahrenstechnik Lukas Hartmann Lehrstuhl für Feststoff- und Grenzflächenverfahrenstechnik

Involved external institutions

PSS Polymer Standards Service GmbH DE Germany (DE)

How to cite

APA:

Peukert, W., Kaspereit, M., Hofe, T., & Gromotka, L. (2022). Size exclusion chromatography (SEC). In Particle Separation Techniques. (pp. 409-447).

MLA:

Peukert, Wolfgang, et al. "Size exclusion chromatography (SEC)." Particle Separation Techniques. 2022. 409-447.

BibTeX: Download