Superparamagnetic Iron Oxide Nanoparticles for Targeted Cell Seeding: Magnetic Patterning and Magnetic 3D Cell Culture
Kappes M, Friedrich B, Pfister F, Huber C, Friedrich RP, Stein R, Braun C, Band J, Schreiber E, Alexiou C, Janko C (2022)
Publication Type: Journal article
Publication year: 2022
Journal
Abstract
In regenerative medicine, noncontact manipulation of cells enables new possibilities for tissue engineering. Due to their physicochemical properties, superparamagnetic iron oxide nanoparticles (SPIONs) are used in biomedicine for various applications, e.g., as drug transporters, contrast agents or to make cells maneuverable by magnetic forces. SPIONs attached to and/or taken up by cells enable their magnetic targeting for adoptive immune therapies or tissue engineering. Remote control of different “magnetized” cell types can be used to construct multilayered tissues without the need for a scaffold structure. Here, the suitability of SPIONs with various coatings, such as polyacrylic acid-co-maleic acid (PAM), lauric acid (LA), lauric acid-human serum albumin (HSA), and citrate to magnetize cells is compared with the commercially available NanoShuttle-PL, designed for use in magnetic 3D cell cultures. Depending on the amount of cellular labeling, magnetic control is more or less effective. In particular, PAM- and citrate-coated SPIONs achieve good cellular loading and provide magnetic controllability of cells. In 2D cell culture, the magnetic cargo allows the patterned culture of cells. In 3D, SPIONs enable and accelerate spheroid formation as well as micropatterning using unloaded and loaded cells in parallel.
Authors with CRIS profile
Bernhard Friedrich
Department of Otorhinolaryngology – Head and Neck Surgery
Felix Pfister
Department of Otorhinolaryngology – Head and Neck Surgery
Christian Huber
Department of Otorhinolaryngology – Head and Neck Surgery
René Stein
Department of Otorhinolaryngology – Head and Neck Surgery
Christoph Alexiou
Professur für Nanomedizin
Christina Janko
Department of Otorhinolaryngology – Head and Neck Surgery
Involved external institutions
Germany (DE)How to cite
APA:
Kappes, M., Friedrich, B., Pfister, F., Huber, C., Friedrich, R.P., Stein, R.,... Janko, C. (2022). Superparamagnetic Iron Oxide Nanoparticles for Targeted Cell Seeding: Magnetic Patterning and Magnetic 3D Cell Culture. Advanced Functional Materials. https://doi.org/10.1002/adfm.202203672
MLA:
Kappes, Mona, et al. "Superparamagnetic Iron Oxide Nanoparticles for Targeted Cell Seeding: Magnetic Patterning and Magnetic 3D Cell Culture." Advanced Functional Materials (2022).
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