Systematic Investigation of CMTM Family Genes Suggests Relevance to Glioblastoma Pathogenesis and CMTM1 and CMTM3 as Priority Targets

Delic S, Thuy A, Schulze M, Proescholdt MA, Dietrich P, Boßerhoff AK, Riemenschneider MJ (2015)

Publication Type: Journal article

Publication year: 2015

Journal

Genes Chromosomes & Cancer Wiley-Blackwell

Book Volume: 54

Pages Range: 433-443

Journal Issue: 7

DOI: 10.1002/gcc.22255

Abstract

The novel CKLF-like Marvel Transmembrane Domain-containing gene family (CMTM) consists of 8 members (CMTM1-8). As little is known about the oncogenic impact of these genes, we aimed to systematically investigate the relevance of CMTMs to glioblastoma pathogenesis. We performed mRNA expression analyses and survival correlations in glioblastoma patients. Moreover, we analyzed the impact of RNAi-based silencing and overexpression of CMTM family genes on tumor cell proliferation and invasion in vitro. CMTMs appeared to be widely regulated in the group of glioblastomas relative to non-neoplastic brain (NB) tissue (significant upregulation for CMTM2, 3, and 6 and significant downregulation for CMTM 4 and 8). For CMTM1, 5 and 7, we found aberrant expression levels in individual tumors. Functionally, CMTM1, 3, and 7 promoted tumor cell invasion, while CMTM1 additionally enhanced cell proliferation. In a large clinically annotated dataset, higher CMTM1 and 3 expression was significantly correlated with shorter overall survival. Our data thus suggest CMTM1 and 3 as priority targets in glioblastomas. Using a human phosphokinase protein expression profiling assay, we can provide first insights into signalling of these two genes that might be conveyed by growth factor receptor, Src family kinase and WNT activation.

Authors with CRIS profile

Peter Dietrich Department of Medicine 1 – Gastroenterology, Pneumology and Endocrinology Anja Katrin Boßerhoff Lehrstuhl für Biochemie und Molekulare Medizin

Involved external institutions

Universitätsklinikum Regensburg DE Germany (DE)

How to cite

APA:

Delic, S., Thuy, A., Schulze, M., Proescholdt, M.A., Dietrich, P., Boßerhoff, A.K., & Riemenschneider, M.J. (2015). Systematic Investigation of CMTM Family Genes Suggests Relevance to Glioblastoma Pathogenesis and CMTM1 and CMTM3 as Priority Targets. Genes Chromosomes & Cancer, 54(7), 433-443. https://doi.org/10.1002/gcc.22255

MLA:

Delic, Sabit, et al. "Systematic Investigation of CMTM Family Genes Suggests Relevance to Glioblastoma Pathogenesis and CMTM1 and CMTM3 as Priority Targets." Genes Chromosomes & Cancer 54.7 (2015): 433-443.

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