Spray-dried pneumococcal membrane vesicles are promising candidates for pulmonary immunization
Mehanny M, Boese A, Bornamehr B, Hoppstädter J, Presser V, Kiemer AK, Lehr CM, Fuhrmann G (2022)
Publication Type: Journal article
Publication year: 2022
Journal
Book Volume: 621
Article Number: 121794
DOI: 10.1016/j.ijpharm.2022.121794
Abstract
Pneumococcal infections represent a global health threat, which requires novel vaccine developments. Extracellular vesicles are secreted from most cells, including prokaryotes, and harbor virulence factors and antigens. Hence, bacterial membrane vesicles (MVs) may induce a protective immune response. For the first time, we formulate spray-dried gram-positive pneumococcal MVs-loaded vaccine microparticles using lactose/leucine as inert carriers to enhance their stability and delivery for pulmonary immunization. The optimized vaccine microparticles showed a mean particle size of 1–2 µm, corrugated surface, and nanocrystalline nature. Their aerodynamic diameter of 2.34 µm, average percentage emitted dose of 88.8%, and fine powder fraction 79.7%, demonstrated optimal flow properties for deep alveolar delivery using a next-generation impactor. Furthermore, confocal microscopy confirmed the successful encapsulation of pneumococcal MVs within the prepared microparticles. Human macrophage-like THP-1 cells displayed excellent viability, negligible cytotoxicity, and a rapid uptake around 60% of fluorescently labeled MVs after incubation with vaccine microparticles. Moreover, vaccine microparticles increased the release of pro-inflammatory cytokines tumor necrosis factor and interleukin-6 from primary human peripheral blood mononuclear cells. Vaccine microparticles exhibited excellent properties as promising vaccine candidates for pulmonary immunization and are optimal for further animal testing, scale-up and clinical translation.
Authors with CRIS profile
Involved external institutions
Leibniz-Institut für Neue Materialien gGmbH / Leibniz Institute for New Materials (INM)
Germany (DE)
Universität des Saarlandes (UdS)
Germany (DE)
Helmholtz-Institut für Pharmazeutische Forschung Saarland (HIPS)
Germany (DE)
Germany (DE)
Universität des Saarlandes (UdS)
Germany (DE)
Helmholtz-Institut für Pharmazeutische Forschung Saarland (HIPS)
Germany (DE)
How to cite
APA:
Mehanny, M., Boese, A., Bornamehr, B., Hoppstädter, J., Presser, V., Kiemer, A.K.,... Fuhrmann, G. (2022). Spray-dried pneumococcal membrane vesicles are promising candidates for pulmonary immunization. International Journal of Pharmaceutics, 621. https://dx.doi.org/10.1016/j.ijpharm.2022.121794
MLA:
Mehanny, Mina, et al. "Spray-dried pneumococcal membrane vesicles are promising candidates for pulmonary immunization." International Journal of Pharmaceutics 621 (2022).
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