Multiphoton Microscopy Reveals DAPK1‐Dependent Extracellular Matrix Remodeling in a Chorioallantoic Membrane (CAM) Model

Kunze P, Kreiß L, Novosadová V, Roehe AV, Steinmann S, Prochazka J, Geppert CI, Hartmann A, Schürmann S, Friedrich O, Schneider-Stock R (2022)

Publication Type: Journal article, Original article

Publication year: 2022

Journal

Cancers MDPI

Book Volume: 14

Article Number: 2364

Journal Issue: 10

DOI: 10.3390/cancers14102364

Abstract

Cancer cells facilitate tumor growth by creating favorable tumor micro‐environments (TME), altering homeostasis and immune response in the extracellular matrix (ECM) of surrounding tissue. A potential factor that contributes to TME generation and ECM remodeling is the cytoskeleton‐associated human death‐associated protein kinase 1 (DAPK1). Increased tumor cell motility and de‐adhesion (thus, promoting metastasis), as well as upregulated plasminogen-signaling, are shown when functionally analyzing the DAPK1 ko‐related proteome. However, the systematic investigation of how tumor cells actively modulate the ECM at the tissue level is experimentally challenging since animal models do not allow direct experimental access while artificial in vitro scaffolds cannot simulate the entire complexity of tissue systems. Here, we used the chorioallantoic membrane (CAM) assay as a natural, collagen‐rich tissue model in combination with all‐optical experimental access by multiphoton microscopy (MPM) to study the ECM remodeling potential of colorectal tumor cells with and without DAPK1 in situ and even in vivo. This approach demonstrates the suitability of the CAM assay in combination with multiphoton microscopy for studying collagen remodeling during tumor growth. Our results indicate the high ECM remodeling potential of DAPK1 ko tumor cells at the tissue level and support our findings from proteomics.

Authors with CRIS profile

Philipp Kunze Institute of Pathology Lucas Kreiß Lehrstuhl für Medizinische Biotechnologie Sara Steinmann Institute of Pathology Carol-Immanuel Geppert Institute of Pathology Arndt Hartmann Lehrstuhl für Allgemeine Pathologie und Pathologische Anatomie Sebastian Schürmann Lehrstuhl für Medizinische Biotechnologie Oliver Friedrich Lehrstuhl für Medizinische Biotechnologie Regine Schneider-Stock Professur für Experimentelle Tumorpathologie

Involved external institutions

Academy of Sciences of the Czech Republic (ASCR) / Akademie věd České republiky (AVČR) CZ Czech Republic (CZ) Universidade Federal de Ciências da Saúde de Porto Alegre BR Brazil (BR)

How to cite

APA:

Kunze, P., Kreiß, L., Novosadová, V., Roehe, A.V., Steinmann, S., Prochazka, J.,... Schneider-Stock, R. (2022). Multiphoton Microscopy Reveals DAPK1‐Dependent Extracellular Matrix Remodeling in a Chorioallantoic Membrane (CAM) Model. Cancers, 14(10). https://doi.org/10.3390/cancers14102364

MLA:

Kunze, Philipp, et al. "Multiphoton Microscopy Reveals DAPK1‐Dependent Extracellular Matrix Remodeling in a Chorioallantoic Membrane (CAM) Model." Cancers 14.10 (2022).

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