Soft Polydimethylsiloxane-Supported Lipid Bilayers for Studying T Cell Interactions.
Lippert AH, Dimov IB, Winkel AK, Humphrey J, Mccoll J, Chen KY, Santos AM, Jenkins E, Franze K, Davis SJ, Klenerman D (2021)
Publication Type: Journal article
Publication year: 2021
Journal
Book Volume: 120
Pages Range: 35-45
Journal Issue: 1
DOI: 10.1016/j.bpj.2020.11.021
Abstract
Much of what we know about the early stages of T cell activation has been obtained from studies of T cells interacting with glass-supported lipid bilayers that favor imaging but are orders of magnitude stiffer than typical cells. We developed a method for attaching lipid bilayers to polydimethylsiloxane polymer supports, producing "soft bilayers" with physiological levels of mechanical resistance (Young's modulus of 4 kPa). Comparisons of T cell behavior on soft and glass-supported bilayers revealed that whereas late stages of T cell activation are thought to be substrate-stiffness dependent, early calcium signaling was unaffected by substrate rigidity, implying that early steps in T cell receptor triggering are not mechanosensitive. The exclusion of large receptor-type phosphatases was observed on the soft bilayers, however, even though it is yet to be demonstrated at authentic cell-cell contacts. This work sets the stage for an imaging-based exploration of receptor signaling under conditions closely mimicking physiological cell-cell contact.
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APA:
Lippert, A.H., Dimov, I.B., Winkel, A.K., Humphrey, J., Mccoll, J., Chen, K.Y.,... Klenerman, D. (2021). Soft Polydimethylsiloxane-Supported Lipid Bilayers for Studying T Cell Interactions. Biophysical Journal, 120(1), 35-45. https://dx.doi.org/10.1016/j.bpj.2020.11.021
MLA:
Lippert, Anna H., et al. "Soft Polydimethylsiloxane-Supported Lipid Bilayers for Studying T Cell Interactions." Biophysical Journal 120.1 (2021): 35-45.
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