Concise report: a minimal-invasive method to retrieve and identify entheseal tissue from psoriatic arthritis patients

Pachowsky M, Raimondo MG, Xu C, Rauber S, Tascilar K, Labinsky H, Vogg M, Saad Msa, Simon D, Rech J, Soare A, Bräuer L, Kleyer A, Schett G, Ramming A (2022)

Publication Type: Journal article

Publication year: 2022

Journal

Annals of the Rheumatic Diseases BMJ Publishing Group

DOI: 10.1136/annrheumdis-2021-222061

Abstract

Objectives To establish a minimally invasive biopsy technique for the analysis of entheseal tissue in patients with psoriatic arthritis (PsA). Methods Human cadavers were used for establishing the technique to retrieve tissue from the lateral humeral epicondyle enthesis (cadaveric biopsies). After biopsy, the entire enthesis was surgically resected (cadaveric resections). Biopsies and resections were assessed by label-free second harmonic generation (SHG) microscopy. The same technique was then applied in patients with PsA with definition of entheseal tissue by SHG, staining of CD45+immune cells and RNA extraction. Results Entheseal biopsies from five cadavers allowed the retrieval of entheseal tissue as validated by the analysis of resection material. Microscopy of biopsy and resection sections allowed differentiation of entheseal, tendon and muscle tissue by SHG and definition of specific intensity thresholds for entheseal tissue. In subsequent entheseal biopsies of 10 PsA patients: the fraction of entheseal tissue was high (65%) and comparable to cadaveric biopsies (68%) as assessed by SHG microscopy. Furthermore, PsA biopsies showed immune cell infiltration and sufficient retrieval of RNA for further molecular analysis. Conclusion Entheseal biopsy of the lateral epicondyle is feasible in patients with PsA allowing reliable retrieval of entheseal tissue and its identification by SHG microscopy.

Authors with CRIS profile

Milena Pachowsky Department of Medicine 3 – Rheumatology and Immunology Maria Gabriella Raimondo Department of Medicine 3 – Rheumatology and Immunology Cong Xu Department of Medicine 3 – Rheumatology and Immunology Simon Rauber Department of Medicine 3 – Rheumatology and Immunology Koray Tascilar Department of Medicine 3 – Rheumatology and Immunology Hannah Labinsky Department of Medicine 3 – Rheumatology and Immunology Mario Raphael Angeli Department of Medicine 3 – Rheumatology and Immunology Mina saad aziz Saad Department of Medicine 3 – Rheumatology and Immunology David Simon Department of Medicine 3 – Rheumatology and Immunology Hans Jürgen Rech Department of Medicine 3 – Rheumatology and Immunology Lars Bräuer Professur für Oberflächenaktive Proteine Arnd Kleyer Department of Medicine 3 – Rheumatology and Immunology Georg Schett Lehrstuhl für Innere Medizin III Andreas Ramming Medizinische Fakultät

How to cite

APA:

Pachowsky, M., Raimondo, M.G., Xu, C., Rauber, S., Tascilar, K., Labinsky, H.,... Ramming, A. (2022). Concise report: a minimal-invasive method to retrieve and identify entheseal tissue from psoriatic arthritis patients. Annals of the Rheumatic Diseases. https://doi.org/10.1136/annrheumdis-2021-222061

MLA:

Pachowsky, Milena, et al. "Concise report: a minimal-invasive method to retrieve and identify entheseal tissue from psoriatic arthritis patients." Annals of the Rheumatic Diseases (2022).

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