Boltz F, Enders M, Feigenspan A, Kirchner P, Ekici AB, Kürten S (2022)
Publication Type: Journal article
Publication year: 2022
Book Volume: 12
Article Number: 476
Journal Issue: 4
Multiple sclerosis (MS) is a chronic autoimmune disease of the central nervous system (CNS). Therapy is currently limited to drugs that interfere with the immune system; treatment options that primarily mediate neuroprotection and prevent neurodegeneration are not available. Here, we studied the effects of nimodipine on the rat cell line OLN-93, which resembles young mature oligodendrocytes. Nimodipine is a dihydropyridine that blocks the voltage-gated L-type calcium channel family members Cav1.2 and Cav1.3. Our data show that the treatment of OLN-93 cells with nimodipine induced the upregulation of myelin genes, in particular of proteolipid protein 1 (Plp1), which was confirmed by a significantly greater expression of PLP1 in immunofluorescence analysis and the presence of myelin structures in the cytoplasm at the ultrastructural level. Whole-genome RNA sequencing additionally revealed the upregulation of genes that are involved in neuroprotection, remyelination, and antioxidation pathways. Interestingly, the observed effects were independent of Cav1.2 and Cav1.3 because OLN-93 cells do not express these channels, and there was no measurable response pattern in patch-clamp analysis. Taking into consideration previous studies that demonstrated a beneficial effect of nimodipine on microglia, our data support the notion that nimodipine is an interesting drug candidate for the treatment of MS and other demyelinating diseases.
APA:
Boltz, F., Enders, M., Feigenspan, A., Kirchner, P., Ekici, A.B., & Kürten, S. (2022). Nimodipine Exerts Beneficial Effects on the Rat Oligodendrocyte Cell Line OLN-93. Brain Sciences, 12(4). https://doi.org/10.3390/brainsci12040476
MLA:
Boltz, Felix, et al. "Nimodipine Exerts Beneficial Effects on the Rat Oligodendrocyte Cell Line OLN-93." Brain Sciences 12.4 (2022).
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