Nimodipine Exerts Beneficial Effects on the Rat Oligodendrocyte Cell Line OLN-93

Boltz F, Enders M, Feigenspan A, Kirchner P, Ekici AB, Kürten S (2022)


Publication Type: Journal article

Publication year: 2022

Journal

Book Volume: 12

Article Number: 476

Journal Issue: 4

DOI: 10.3390/brainsci12040476

Abstract

Multiple sclerosis (MS) is a chronic autoimmune disease of the central nervous system (CNS). Therapy is currently limited to drugs that interfere with the immune system; treatment options that primarily mediate neuroprotection and prevent neurodegeneration are not available. Here, we studied the effects of nimodipine on the rat cell line OLN-93, which resembles young mature oligodendrocytes. Nimodipine is a dihydropyridine that blocks the voltage-gated L-type calcium channel family members Cav1.2 and Cav1.3. Our data show that the treatment of OLN-93 cells with nimodipine induced the upregulation of myelin genes, in particular of proteolipid protein 1 (Plp1), which was confirmed by a significantly greater expression of PLP1 in immunofluorescence analysis and the presence of myelin structures in the cytoplasm at the ultrastructural level. Whole-genome RNA sequencing additionally revealed the upregulation of genes that are involved in neuroprotection, remyelination, and antioxidation pathways. Interestingly, the observed effects were independent of Cav1.2 and Cav1.3 because OLN-93 cells do not express these channels, and there was no measurable response pattern in patch-clamp analysis. Taking into consideration previous studies that demonstrated a beneficial effect of nimodipine on microglia, our data support the notion that nimodipine is an interesting drug candidate for the treatment of MS and other demyelinating diseases.

Authors with CRIS profile

How to cite

APA:

Boltz, F., Enders, M., Feigenspan, A., Kirchner, P., Ekici, A.B., & Kürten, S. (2022). Nimodipine Exerts Beneficial Effects on the Rat Oligodendrocyte Cell Line OLN-93. Brain Sciences, 12(4). https://doi.org/10.3390/brainsci12040476

MLA:

Boltz, Felix, et al. "Nimodipine Exerts Beneficial Effects on the Rat Oligodendrocyte Cell Line OLN-93." Brain Sciences 12.4 (2022).

BibTeX: Download