Schwann Cells Promote Myogenic Differentiation of Myoblasts and Adipogenic Mesenchymal Stromal Cells on Poly-ɛ-Caprolactone-Collagen I-Nanofibers

Cai A, Zheng Z, Himmler M, Schubert DW, Fuchsluger TA, Weisbach VG, Horch RE, Arkudas A (2022)

Publication Type: Journal article

Publication year: 2022

Journal

Cells MDPI

Book Volume: 11

Pages Range: 1436

Issue: 9

DOI: 10.3390/cells11091436

Abstract

For the purpose of skeletal muscle tissue engineering, different cell types have been investigated regarding their myogenic differentiation potential, including co-cultured myoblasts and adipogenic mesenchymal stromal cells (Mb/ADSC). As neural cells enhance synaptic junction formation, the aim of this study was to co-culture Schwann cells (SCs) with Mb/ADSC on biocompatible electrospun aligned poly-ε-polycaprolacton (PCL)-collagen I-nanofibers. It was hypothesized that SCs, as part of the peripheral nervous system, promote the myogenic differentiation of Mb/ADSC co-cultures. Mb/ADSC were compared to Mb/ADSC/SC regarding their capacity for myogenic differentiation via immunofluorescent staining and gene expression of myogenic markers. Mb/ADSC/SC showed more myotubes after 28 days of differentiation (p ≤ 0.05). After 28 days of differentiation on electrospun aligned PCL-collagen I-nanofibers, gene expression of myosin heavy chains (MYH2) and myogenin (MYOG) was upregulated in Mb/ADSC/SC compared to Mb/ADSC (p ≤ 0.01 and p ≤ 0.05, respectively). Immunofluorescent staining for MHC showed highly aligned multinucleated cells as possible myotube formation in Mb/ADSC/SC. In conclusion, SCs promote myogenic differentiation of Mb/ADSC. The co-culture of primary Mb/ADSC/SC on PCL-collagen I-nanofibers serves as a physiological model for skeletal muscle tissue engineering, applicable to future clinical applications.

Authors with CRIS profile

Aijia Cai Department of Plastic and Hand Surgery Zengming Zheng Department of Plastic and Hand Surgery Marcus Himmler Lehrstuhl für Werkstoffwissenschaften (Polymerwerkstoffe) Dirk Schubert Lehrstuhl für Werkstoffwissenschaften (Polymerwerkstoffe) Volker Günter Weisbach Medizinische Fakultät Raymund Horch Professur für Plastische Chirurgie und Handchirurgie Andreas Arkudas Medizinische Fakultät

Additional Organisation(s)

Lehrstuhl für Werkstoffwissenschaften (Polymerwerkstoffe)

Involved external institutions

Universitätsmedizin Rostock DE Germany (DE)

How to cite

APA:

Cai, A., Zheng, Z., Himmler, M., Schubert, D.W., Fuchsluger, T.A., Weisbach, V.G.,... Arkudas, A. (2022). Schwann Cells Promote Myogenic Differentiation of Myoblasts and Adipogenic Mesenchymal Stromal Cells on Poly-ɛ-Caprolactone-Collagen I-Nanofibers. Cells, 11, 1436. https://dx.doi.org/10.3390/cells11091436

MLA:

Cai, Aijia, et al. "Schwann Cells Promote Myogenic Differentiation of Myoblasts and Adipogenic Mesenchymal Stromal Cells on Poly-ɛ-Caprolactone-Collagen I-Nanofibers." Cells 11 (2022): 1436.

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