Macrophage‐like Cells Are Responsive to Titania Nanotube Intertube Spacing—An In Vitro Study

Necula MG, Mazare AV, Negrescu AM, Mitran V, Özkan S, Trusca R, Park J, Schmuki P, Cimpean A (2022)

Publication Type: Journal article

Publication year: 2022

Journal

International Journal of Molecular Sciences MDPI

Book Volume: 23

Article Number: 3558

Journal Issue: 7

DOI: 10.3390/ijms23073558

Abstract

With the introduction of a new interdisciplinary field, osteoimmunology, today, it is well acknowledged that biomaterial‐induced inflammation is modulated by immune cells, primarily macrophages, and can be controlled by nanotopographical cues. Recent studies have investigated the effect of surface properties in modulating the immune reaction, and literature data indicate that various surface cues can dictate both the immune response and bone tissue repair. In this context, the purpose of the present study was to investigate the effects of titanium dioxide nanotube (TNT) interspacing on the response of the macrophage‐like cell line RAW 264.7. The cells were maintained in contact with the surfaces of flat titanium (Ti) and anodic TNTs with an intertube spacing of 20 nm (TNT20) and 80 nm (TNT80), under standard or pro‐inflammatory conditions. The results revealed that nanotube interspacing can influence macrophage response in terms of cell survival and proliferation, cellular morphology and polarization, cytokine/chemokine expression, and foreign body reaction. While the nanostructured topography did not tune the macrophages’ differentiation into osteoclasts, this behavior was significantly reduced as compared to flat Ti surface. Overall, this study provides a new insight into how nanotubes’ morphological features, particularly intertube spacing, could affect macrophage behavior.

Authors with CRIS profile

Anca Valentina Mazare Lehrstuhl für Werkstoffwissenschaften (Korrosion und Oberflächentechnik) Selda Özkan Lehrstuhl für Werkstoffwissenschaften (Korrosion und Oberflächentechnik) Patrik Schmuki Lehrstuhl für Werkstoffwissenschaften (Korrosion und Oberflächentechnik)

Involved external institutions

University of Bucharest / Universitatea din București RO Romania (RO) Politechnic University of Bucharest / Universitatea Politehnica din București RO Romania (RO)

How to cite

APA:

Necula, M.G., Mazare, A.V., Negrescu, A.M., Mitran, V., Özkan, S., Trusca, R.,... Cimpean, A. (2022). Macrophage‐like Cells Are Responsive to Titania Nanotube Intertube Spacing—An In Vitro Study. International Journal of Molecular Sciences, 23(7). https://dx.doi.org/10.3390/ijms23073558

MLA:

Necula, Madalina Georgiana, et al. "Macrophage‐like Cells Are Responsive to Titania Nanotube Intertube Spacing—An In Vitro Study." International Journal of Molecular Sciences 23.7 (2022).

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