Systematic interpretation of confocal laser endomicroscopy: larynx and pharynx confocal imaging score Interpretazione sistematica dell’endomicroscopia confocale laser: punteggio endomicroscopico confocale laringeo e faringeo

Sievert M, Mantsopoulos K, Müller-Hübner S, Eckstein M, Rupp R, Aubreville M, Stelzle F, Oetter N, Maier A, Iro H, Goncalves M (2022)

Publication Type: Journal article

Publication year: 2022

Journal

Acta Otorhinolaryngologica Italica Pacini Editore SpA

Book Volume: 42

Pages Range: 26-33

Journal Issue: 1

DOI: 10.14639/0392-100X-N1643

Abstract

Objective. Development and validation of a confocal laser endomicroscopy (CLE) classification score for the larynx and pharynx. Methods. Thirteen patients (154 video sequences, 9240 images) with laryngeal or phar-yngeal SCC were included in this prospective study between October 2020 and February 2021. Each CLE sequence was correlated with the gold standard of histopathological ex-amination. Based on a dataset of 94 video sequences (5640 images), a scoring system was developed. In the remaining 60 sequences (3600 images), the score was validated by four CLE experts and four head and neck surgeons who were not familiar with CLE. Results. Tissue homogeneity, cell size, borders and clusters, capillary loops and the nu-cleus/cytoplasm ratio were defined as the scoring criteria. Using this score, the CLE experts obtained an accuracy, sensitivity, and specificity of 90.8%, 95.1%, and 86.4%, respectively, and the CLE non-experts of 86.2%, 86.4%, and 86.1%. Interobserver agreement Fleiss’ kappa was 0.8 and 0.6, respectively. Conclusions. CLE can be reliably evaluated based on defined and reproducible imaging features, which demonstrate a high diagnostic value. CLE can be easily integrated into the intraoperative setting and generate real-time, in-vivo microscopic images to demarcate malignant changes.

Authors with CRIS profile

Matti Sievert Department of Otorhinolaryngology – Head and Neck Surgery Konstantinos Mantsopoulos Department of Otorhinolaryngology – Head and Neck Surgery Sarina Müller-Hübner Department of Otorhinolaryngology – Head and Neck Surgery Markus Eckstein Institute of Pathology Robin Rupp Department of Otorhinolaryngology – Head and Neck Surgery Florian Stelzle Department of Oral and Cranio-Maxillofacial Surgery Nicolai Oetter Department of Oral and Cranio-Maxillofacial Surgery Andreas Maier Lehrstuhl für Informatik 5 (Mustererkennung) Heinrich Iro Lehrstuhl für Hals-, Nasen- und Ohrenheilkunde Miguel Goncalves Department of Otorhinolaryngology – Head and Neck Surgery

Involved external institutions

Technische Hochschule Ingolstadt DE Germany (DE)

How to cite

APA:

Sievert, M., Mantsopoulos, K., Müller-Hübner, S., Eckstein, M., Rupp, R., Aubreville, M.,... Goncalves, M. (2022). Systematic interpretation of confocal laser endomicroscopy: larynx and pharynx confocal imaging score Interpretazione sistematica dell’endomicroscopia confocale laser: punteggio endomicroscopico confocale laringeo e faringeo. Acta Otorhinolaryngologica Italica, 42(1), 26-33. https://doi.org/10.14639/0392-100X-N1643

MLA:

Sievert, Matti, et al. "Systematic interpretation of confocal laser endomicroscopy: larynx and pharynx confocal imaging score Interpretazione sistematica dell’endomicroscopia confocale laser: punteggio endomicroscopico confocale laringeo e faringeo." Acta Otorhinolaryngologica Italica 42.1 (2022): 26-33.

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