Degradation and de novo formation of nine major glucose degradation products during storage of peritoneal dialysis fluids

Gensberger-Reigl S, Weigel I, Stützer J, Auditore A, Nikolaus T, Pischetsrieder M (2022)

Publication Type: Journal article

Publication year: 2022

Journal

Book Volume: 12

Article Number: 4268

Journal Issue: 1

DOI: 10.1038/s41598-022-08123-1

Abstract

Reactive glucose degradation products (GDPs) are formed during heat sterilization of glucose-containing peritoneal dialysis fluids (PDFs) and may induce adverse clinical effects. Long periods of storage and/or transport of PDFs before use may lead to de novo formation or degradation of GDPs. Therefore, the present study quantified the GDP profiles of single- and double-chamber PDFs during storage. Glucosone, 3-deoxyglucosone (3-DG), 3-deoxygalactosone (3-DGal), 3,4-dideoxyglucosone-3-ene (3,4-DGE), glyoxal, methylglyoxal (MGO), acetaldehyde, formaldehyde, and 5-hydroxymethylfurfural (5-HMF) were quantified by two validated UHPLC-DAD methods after derivatization with o-phenylenediamine (dicarbonyls) or 2,4-dinitrophenylhydrazine (monocarbonyls). The PDFs were stored at 50 °C for 0, 1, 2, 4, 13, and 26 weeks. The total GDP concentration of single-chamber PDFs did not change considerably during storage (496.6 ± 16.0 µM, 0 weeks; 519.1 ± 13.1 µM, 26 weeks), but individual GDPs were affected differently. 3-DG (− 82.6 µM) and 3-DGal (− 71.3 µM) were degraded, whereas 5-HMF (+ 161.7 µM), glyoxal (+ 32.2 µM), and formaldehyde (+ 12.4 µM) accumulated between 0 and 26 weeks. Acetaldehyde, glucosone, MGO, and 3,4-DGE showed time-dependent formation and degradation. The GDP concentrations in double-chamber fluids were generally lower and differently affected by storage. In conclusion, the changes of GDP concentrations during storage should be considered for the evaluation of clinical effects of PDFs.

Authors with CRIS profile

Involved external institutions

Fresenius Medical Care Deutschland GmbH Germany (DE)

How to cite

APA:

Gensberger-Reigl, S., Weigel, I., Stützer, J., Auditore, A., Nikolaus, T., & Pischetsrieder, M. (2022). Degradation and de novo formation of nine major glucose degradation products during storage of peritoneal dialysis fluids. Scientific Reports, 12(1). https://dx.doi.org/10.1038/s41598-022-08123-1

MLA:

Gensberger-Reigl, Sabrina, et al. "Degradation and de novo formation of nine major glucose degradation products during storage of peritoneal dialysis fluids." Scientific Reports 12.1 (2022).

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