Synthesis and characterization of a collagen-based composite material containing selenium nanoparticles
Stevanović MM, Filipović N, Kuzmanović M, Tomić N, Ušjak D, Milenković M, Zheng K, Stampfl J, Boccaccini AR (2022)
Publication Type: Journal article
Publication year: 2022
Journal
DOI: 10.1177/08853282211073731
Abstract
Multidrug-resistant bacterial strains represent an emerging global health threat and a great obstacle for bone tissue engineering. One of the major components of the extracellular matrix of the bone is a collagen protein, while selenium is an element that has antimicrobial potential, and is also important for bone metabolism and bone health. Here we represent the incorporation of selenium nanoparticles (SeNPs) synthesized by the green chemical reduction method into collagen gels to produce a composite material, collagen/SeNPs, with antimicrobial properties. The samples were comprehensively characterized by zeta potential measurements, dynamic light scattering inductively coupled plasma-mass spectrometry (ICP-MS), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), optical microscopy, field-emission scanning electron microscopy (FE-SEM), and differential scanning calorimetry The cytotoxicity of the SeNPS, as well as collagen/SeNPs, was tested on the MRC-5 cells. It was revealed that collagen/SeNPS expressed a lower cytotoxic effect. Collagen/SeNPs showed significant antibacterial activity against all tested Gram-positive strains, the major causative agents of orthopedic infections as well as Candida albicans. Furthermore, three-dimensional β-tricalcium phosphate (3D-TCP) scaffolds were fabricated by a well-established 3D printing (lithography) method, and afterward preliminary coated by newly-synthesized SeNPs or collagen/SeNPs. In addition, uncoated 3D-TCP scaffolds as well as coated by collagen/SeNPs were subjected to biofilm formation. The production of Staphylococcus aureus biofilm on coated scaffolds by collagen/SeNPs was significantly reduced compared to the uncoated ones.
Authors with CRIS profile
Kai Zheng
Lehrstuhl für Werkstoffwissenschaften (Biomaterialien)
Aldo Boccaccini
Lehrstuhl für Werkstoffwissenschaften (Biomaterialien)
Involved external institutions
Institute of Technical Sciences of the Serbian Academy of Sciences and Arts / Institut tehničkih nauka SANU
Serbia (RS)
University of Belgrade / Универзитет у Београду
Serbia (RS)
Technische Universität Wien / Vienna University of Technology
Austria (AT)
Serbia (RS)
University of Belgrade / Универзитет у Београду
Serbia (RS)
Technische Universität Wien / Vienna University of Technology
Austria (AT)
How to cite
APA:
Stevanović, M.M., Filipović, N., Kuzmanović, M., Tomić, N., Ušjak, D., Milenković, M.,... Boccaccini, A.R. (2022). Synthesis and characterization of a collagen-based composite material containing selenium nanoparticles. Journal of Biomaterials Applications. https://dx.doi.org/10.1177/08853282211073731
MLA:
Stevanović, Magdalena M., et al. "Synthesis and characterization of a collagen-based composite material containing selenium nanoparticles." Journal of Biomaterials Applications (2022).
BibTeX: Download