Impact of Endothelial Progenitor Cells in the Vascularization of Osteogenic Scaffolds

Steiner D, Reinhardt L, Fischer L, Popp V, Körner C, Geppert CI, Bäuerle T, Horch RE, Arkudas A (2022)

Publication Type: Journal article

Publication year: 2022

Journal

Cells MDPI

Book Volume: 11

Article Number: 926

Journal Issue: 6

DOI: 10.3390/cells11060926

Abstract

The microvascular endothelial network plays an important role in osteogenesis, bone regeneration and bone tissue engineering. Endothelial progenitor cells (EPCs) display a high angiogenic and vasculogenic potential. The endothelialization of scaffolds with endothelial progenitor cells supports vascularization and tissue formation. In addition, EPCs enhance the osteogenic differentiation and bone formation of mesenchymal stem cells (MSCs). This study aimed to investigate the impact of EPCs on vascularization and bone formation of a hydroxyapatite (HA) and beta-tricalcium phosphate (ß-TCP)–fibrin scaffold. Three groups were designed: a scaffold-only group (A), a scaffold and EPC group (B), and a scaffold and EPC/MSC group (C). The HA/ß–TCP–fibrin scaffolds were placed in a porous titanium chamber permitting extrinsic vascularization from the surrounding tissue. Additionally, intrinsic vascularization was achieved by means of an arteriovenous loop (AV loop). After 12 weeks, the specimens were explanted and investigated by histology and CT. We were able to prove a strong scaffold vascularization in all groups. No differences regarding the vessel number and density were detected between the groups. Moreover, we were able to prove bone formation in the coimplantation group. Taken together, the AV loop is a powerful tool for vascularization which is independent from scaffold cellularization with endothelial progenitor cells’ prior implantation.

Authors with CRIS profile

Dominik Steiner Department of Plastic and Hand Surgery Laura Fischer Department of Plastic and Hand Surgery Vanessa Popp Institute of Radiology Carolin Körner Lehrstuhl für Werkstoffwissenschaften (Werkstoffkunde und Technologie der Metalle) Carol-Immanuel Geppert Institute of Pathology Tobias Bäuerle Professur für Multimodale Bildgebung in der präklinischen Forschung Raymund Horch Professur für Plastische Chirurgie und Handchirurgie Andreas Arkudas Professur für Plastische Chirurgie und Handchirurgie

How to cite

APA:

Steiner, D., Reinhardt, L., Fischer, L., Popp, V., Körner, C., Geppert, C.-I.,... Arkudas, A. (2022). Impact of Endothelial Progenitor Cells in the Vascularization of Osteogenic Scaffolds. Cells, 11(6). https://dx.doi.org/10.3390/cells11060926

MLA:

Steiner, Dominik, et al. "Impact of Endothelial Progenitor Cells in the Vascularization of Osteogenic Scaffolds." Cells 11.6 (2022).

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