Current Methods to Unravel the Functional Properties of Lysosomal Ion Channels and Transporters

Festa M, Minicozzi V, Boccaccio A, Lagostena L, Gradogna A, Qi T, Costa A, Larisch N, Hamamoto S, Pedrazzini E, Milenkovic S, Scholz-Starke J, Ceccarelli M, Vitale A, Dietrich P, Uozumi N, Gambale F, Carpaneto A (2022)

Publication Type: Journal article

Publication year: 2022

Journal

Cells MDPI

Book Volume: 11

Article Number: 921

Journal Issue: 6

DOI: 10.3390/cells11060921

Abstract

A distinct set of channels and transporters regulates the ion fluxes across the lysosomal membrane. Malfunctioning of these transport proteins and the resulting ionic imbalance is involved in various human diseases, such as lysosomal storage disorders, cancer, as well as metabolic and neurodegenerative diseases. As a consequence, these proteins have stimulated strong interest for their suitability as possible drug targets. A detailed functional characterization of many lysosomal channels and transporters is lacking, mainly due to technical difficulties in applying the standard patch-clamp technique to these small intracellular compartments. In this review, we focus on current methods used to unravel the functional properties of lysosomal ion channels and transporters, stressing their advantages and disadvantages and evaluating their fields of applicability.

Authors with CRIS profile

Petra Dietrich Professur für Zellbiologie der Pflanzen

Involved external institutions

University of Padua / Universita degli Studi di Padova IT Italy (IT) Università degli Studi di Roma 'Tor Vergata' IT Italy (IT) Consiglio Nazionale delle Ricerche (CNR) / National Research Council of Italy IT Italy (IT) University of Genova / Università degli Studi di Genova IT Italy (IT) Università degli studi di Milano IT Italy (IT) University of Tokyo JP Japan (JP) Tohoku University JP Japan (JP) Università degli Studi di Cagliari IT Italy (IT)

How to cite

APA:

Festa, M., Minicozzi, V., Boccaccio, A., Lagostena, L., Gradogna, A., Qi, T.,... Carpaneto, A. (2022). Current Methods to Unravel the Functional Properties of Lysosomal Ion Channels and Transporters. Cells, 11(6). https://dx.doi.org/10.3390/cells11060921

MLA:

Festa, Margherita, et al. "Current Methods to Unravel the Functional Properties of Lysosomal Ion Channels and Transporters." Cells 11.6 (2022).

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