The anti-fibrotic effect of cold atmospheric plasma on localized scleroderma in vitro and in vivo

Arndt S, Unger P, Boßerhoff AK, Berneburg M, Karrer S (2022)

Publication Type: Conference contribution

Publication year: 2022

Journal

Experimental Dermatology Wiley

Publisher: WILEY

City/Town: HOBOKEN

Pages Range: E83-E83

Conference Proceedings Title: EXPERIMENTAL DERMATOLOGY

DOI: 10.3390/biomedicines9111545

Abstract

Cold Atmospheric Plasma (CAP) has shown promising results in the treatment of various skin diseases. The therapeutic effect of CAP on localized scleroderma (LS), however, has not yet been evaluated. We investigated the effects of CAP on LS by comparing human normal fibroblasts (hNF), human TGF-β-activated fibroblasts (hAF), and human localized scleroderma-derived fibroblasts (hLSF) after direct CAP treatment, co-cultured with plasma-treated human epidermal keratinocytes (hEK) and with an experimental murine model of scleroderma. In hAF and hLSF, 2 min CAP treatment with the MicroPlaSterβ® plasma torch did not affect pro-fibrotic gene expression of alpha smooth muscle actin, fibroblast activating protein, and collagen type I, however, it promoted re-expression of matrix metalloproteinase 1. Functionally, CAP treatment reduced cell migration and stress fiber formation in hAF and hLSF. The relevance of CAP treatment was confirmed in an in vivo model of bleomycin-induced dermal fibrosis. In this model, CAP-treated mice showed significantly reduced dermal thickness and collagen deposition as well as a decrease in both alpha smooth muscle actin-positive myofibroblasts and CD68-positive macrophages in the affected skin in comparison to untreated fibrotic tissue. In conclusion, this study provides the first evidence for the successful use of CAP for treating LS and may be the basis for clinical trials including patients with LS.

Authors with CRIS profile

Anja Katrin Boßerhoff Lehrstuhl für Biochemie und Molekulare Medizin

Involved external institutions

Universitätsklinikum Regensburg DE Germany (DE)

How to cite

APA:

Arndt, S., Unger, P., Boßerhoff, A.K., Berneburg, M., & Karrer, S. (2022). The anti-fibrotic effect of cold atmospheric plasma on localized scleroderma in vitro and in vivo. In EXPERIMENTAL DERMATOLOGY (pp. E83-E83). HOBOKEN: WILEY.

MLA:

Arndt, Stephanie, et al. "The anti-fibrotic effect of cold atmospheric plasma on localized scleroderma in vitro and in vivo." Proceedings of the EXPERIMENTAL DERMATOLOGY HOBOKEN: WILEY, 2022. E83-E83.

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