A Self-Assembled Matrix System for Cell-Bioengineering Applications in Different Dimensions, Scales, and Geometries

Xu Y, Gaillez MP, Zheng K, Voigt D, Cui M, Kurth T, Xiao L, Rothe R, Hauser S, Lee PW, Wieduwild R, Lin W, Bornhauser M, Pietzsch J, Boccaccini AR, Zhang Y (2022)

Publication Type: Journal article

Publication year: 2022

Journal

Small Wiley-VCH Verlag

DOI: 10.1002/smll.202104758

Abstract

Stem cell bioengineering and therapy require different model systems and materials in different stages of development. If a chemically defined biomatrix system can fulfill most tasks, it can minimize the discrepancy among various setups. By screening biomaterials synthesized through a coacervation-mediated self-assembling mechanism, a biomatrix system optimal for 2D human mesenchymal stromal cell (hMSC) culture and osteogenesis is identified. Its utility for hMSC bioengineering is further demonstrated in coating porous bioactive glass scaffolds and nanoparticle synthesis for esiRNA delivery to knock down the SOX-9 gene with high delivery efficiency. The self-assembled injectable system is further utilized for 3D cell culture, segregated co-culture of hMSC with human umbilical vein endothelial cells (HUVEC) as an angiogenesis model, and 3D bioprinting. Most interestingly, the coating of bioactive glass with the self-assembled biomatrix not only supports the proliferation and osteogenesis of hMSC in the 3D scaffold but also induces the amorphous bioactive glass (BG) scaffold surface to form new apatite crystals resembling bone-shaped plate structures. Thus, the self-assembled biomatrix system can be utilized in various dimensions, scales, and geometries for many different bioengineering applications.

Authors with CRIS profile

Kai Zheng Lehrstuhl für Werkstoffwissenschaften (Biomaterialien) Aldo Boccaccini Lehrstuhl für Werkstoffwissenschaften (Biomaterialien)

Involved external institutions

Technische Universität Dresden DE Germany (DE) Wuhan University CN China (CN) Helmholtz-Zentrum Dresden-Rossendorf (HZDR) DE Germany (DE)

How to cite

APA:

Xu, Y., Gaillez, M.P., Zheng, K., Voigt, D., Cui, M., Kurth, T.,... Zhang, Y. (2022). A Self-Assembled Matrix System for Cell-Bioengineering Applications in Different Dimensions, Scales, and Geometries. Small. https://dx.doi.org/10.1002/smll.202104758

MLA:

Xu, Yong, et al. "A Self-Assembled Matrix System for Cell-Bioengineering Applications in Different Dimensions, Scales, and Geometries." Small (2022).

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