Interaction of storage carbohydrates and other cyclic fluxes with central metabolism: A quantitative approach by non-stationary 13C metabolic flux analysis

Suarez-Mendez CA, Hanemaaijer M, ten Pierick A, Wolters JC, Heijnen JJ, Wahl SA (2016)

Publication Type: Journal article

Publication year: 2016

Journal

Metabolic Engineering Communications Elsevier

Book Volume: 3

Pages Range: 52-63

DOI: 10.1016/j.meteno.2016.01.001

Abstract

13C labeling experiments in aerobic glucose limited cultures of Saccharomyces cerevisiae at four different growth rates (0.054; 0.101, 0.207, 0.307 h-1) are used for calculating fluxes that include intracellular cycles (e.g., storage carbohydrate cycles, exchange fluxes with amino acids), which are rearranged depending on the growth rate. At low growth rates the impact of the storage carbohydrate recycle is relatively more significant than at high growth rates due to a higher concentration of these materials in the cell (up to 560-fold) and higher fluxes relative to the glucose uptake rate (up to 16%). Experimental observations suggest that glucose can be exported to the extracellular space, and that its source is related to storage carbohydrates, most likely via the export and subsequent extracellular breakdown of trehalose. This hypothesis is strongly supported by 13C-labeling experimental data, measured extracellular trehalose, and the corresponding flux estimations.

Authors with CRIS profile

Sebastian Aljoscha Wahl

Involved external institutions

Delft University of Technology (TU Delft) NL Netherlands (NL) University of Groningen / Rijksuniversiteit Groningen NL Netherlands (NL)

How to cite

APA:

Suarez-Mendez, C.A., Hanemaaijer, M., ten Pierick, A., Wolters, J.C., Heijnen, J.J., & Wahl, S.A. (2016). Interaction of storage carbohydrates and other cyclic fluxes with central metabolism: A quantitative approach by non-stationary 13C metabolic flux analysis. Metabolic Engineering Communications, 3, 52-63. https://dx.doi.org/10.1016/j.meteno.2016.01.001

MLA:

Suarez-Mendez, C. A., et al. "Interaction of storage carbohydrates and other cyclic fluxes with central metabolism: A quantitative approach by non-stationary 13C metabolic flux analysis." Metabolic Engineering Communications 3 (2016): 52-63.

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