RanGAP1 is a continuous marker of the Arabidopsis cell division plane

Xu XM, Zhao Q, Rodrigo-Peiris T, Brkljacic J, He CS, Mueller S, Meier I (2008)

Publication Type: Journal article

Publication year: 2008

Journal

Proceedings of the National Academy of Sciences of the United States of America National Academy of Sciences

Book Volume: 105

Pages Range: 18637-18642

Journal Issue: 47

DOI: 10.1073/pnas.0806157105

Abstract

In higher plants, the plane of cell division is faithfully predicted by the preprophase band (PPB). The PPB, a cortical ring of microtubules and F-actin, disassembles upon nuclear-envelope breakdown. During cytokinesis, the expanding cell plate fuses with the plasma membrane at the cortical division site, the site of the former PPB. The nature of the "molecular memory" that is left behind by the PPB and is proposed to guide the cell plate to the cortical division site is unknown. RanGAP is the GTPase activating protein of the small GTPase Ran, which provides spatial information for nucleocytoplasmic transport and various mitotic processes in animals. Here, we show that, in dividing root cells, Arabidopsis RanGAP1 concentrates at the PPB and remains associated with the cortical division site during mitosis and cytokinesis, requiring its N-terminal targeting domain. In a fass/ton2 mutant, which affects PPB formation, RanGAP1 recruitment to the PPB site is lost, while its PPB retention is microtubule-independent. RanGAP1 persistence at the cortical division site, but not its initial accumulation at the PPB requires the 2 cytokinesis-regulating kinesins POK1 and POK2. Depletion of RanGAP by inducible RNAi leads to oblique cell walls and cell-wall stubs in root cell files, consistent with cytokinesis defects. We propose that Arabidopsis RanGAP, a continuous positive protein marker of the plant division plane, has a role in spatial signaling during plant cell division. © 2008 by The National Academy of Sciences of the USA.

Authors with CRIS profile

Sabine Müller

Involved external institutions

Ohio State University US United States (USA) (US) University of Auckland NZ New Zealand (NZ)

How to cite

APA:

Xu, X.M., Zhao, Q., Rodrigo-Peiris, T., Brkljacic, J., He, C.S., Mueller, S., & Meier, I. (2008). RanGAP1 is a continuous marker of the Arabidopsis cell division plane. Proceedings of the National Academy of Sciences of the United States of America, 105(47), 18637-18642. https://dx.doi.org/10.1073/pnas.0806157105

MLA:

Xu, Xianfeng Morgan, et al. "RanGAP1 is a continuous marker of the Arabidopsis cell division plane." Proceedings of the National Academy of Sciences of the United States of America 105.47 (2008): 18637-18642.

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