The plant microtubule-associated protein AtMAP65-3/PLE is essential for cytokinetic phragmoplast function
Müller S, Smertenko A, Wagner V, Heinrich M, Hussey PJ, Hauser MT (2004)
Publication Type: Journal article
Publication year: 2004
Journal
Book Volume: 14
Pages Range: 412-417
Journal Issue: 5
DOI: 10.1016/j.cub.2004.02.032
Abstract
Directional cell expansion in interphase and nuclear and cell division in M-phase are mediated by four microtubule arrays, three of which are unique to plants: the interphase array, the preprophase band, and the phragmoplast. The plant microtubule-associated protein MAP65 has been identified as a key structural component in these arrays [1]. The Arabidopsis genome has nine MAP65 genes, and here we show that one, AtMAP65-3/PLE, locates only to the mitotic arrays and is essential for cytokinesis. The Arabidopsis pleiade (ple) alleles are single recessive mutations, and we show that these mutations are in the AtMAP65-3 gene. Moreover, these mutations cause C-terminal truncations that abolish microtubule binding. In the ple mutants the anaphase spindle is normal, and the cytokinetic phragmoplast can form but is distorted; not only is it wider, but the midline, the region where oppositely oriented microtubules overlap, is unusually expanded. Here we present data that demonstrate an essential role for AtMAP65-3/PLE in cytokinesis in plant cells.
Authors with CRIS profile
Involved external institutions
Durham University
United Kingdom (GB)
Universität für Bodenkultur Wien (BOKU) / University of Natural Resources and Life Sciences, Vienna
Austria (AT)
United Kingdom (GB)
Universität für Bodenkultur Wien (BOKU) / University of Natural Resources and Life Sciences, Vienna
Austria (AT)
How to cite
APA:
Müller, S., Smertenko, A., Wagner, V., Heinrich, M., Hussey, P.J., & Hauser, M.-T. (2004). The plant microtubule-associated protein AtMAP65-3/PLE is essential for cytokinetic phragmoplast function. Current Biology, 14(5), 412-417. https://doi.org/10.1016/j.cub.2004.02.032
MLA:
Müller, Sabine, et al. "The plant microtubule-associated protein AtMAP65-3/PLE is essential for cytokinetic phragmoplast function." Current Biology 14.5 (2004): 412-417.
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