PAR-6 levels are regulated by NOS-3 in a CUL-2 dependent manner in Caenorhabditis elegans

Pacquelet A, Zanin E, Ashiono C, Gotta M (2008)

Publication Status: Published

Publication Type: Journal article

Publication year: 2008

Journal

Developmental Biology Elsevier

Publisher: ACADEMIC PRESS INC ELSEVIER SCIENCE

Book Volume: 319

Pages Range: 267-272

Journal Issue: 2

DOI: 10.1016/j.ydbio.2008.04.016

Abstract

The PAR proteins have an essential and conserved function in establishing polarity in many cell types and organisms. However, their key upstream regulators remain to be identified. In C. elegans, regulators of the PAR proteins can be identified by their ability to suppress the lethality of par-2 mutant embryos. Here we show that a nos-3 loss of function mutant suppresses the lethality of par-2 mutants by regulating PAR-6 protein levels. The suppression requires the activity of the sex determination genes fem-1/2/3 and of the cullin cul-2. FEM-1 is a substrate-specific adaptor for a CUL-2-based ubiquitin ligase (CBCFEM-1). Interestingly, we find that CUL-2 is required for the regulation of PAR-6 levels and that PAR-6 physically interacts with FEM-1. Our data strongly suggest that PAR-6 levels are regulated by the CBCFEM-1 ubiquitin ligase thereby uncovering a novel role for the FEM proteins and cullin-dependent degradation in regulating PAR proteins and polarity processes. (c) 2008 Elsevier Inc. All rights reserved.

Authors with CRIS profile

Esther Zanin

Additional Organisation(s)

Professur für Experimentelle Molekulare Zelldynamik

Involved external institutions

Eidgenössische Technische Hochschule Zürich (ETHZ) / Swiss Federal Institute of Technology in Zurich CH Switzerland (CH) University of Geneva / Université de Genève (UNIGE) CH Switzerland (CH)

How to cite

APA:

Pacquelet, A., Zanin, E., Ashiono, C., & Gotta, M. (2008). PAR-6 levels are regulated by NOS-3 in a CUL-2 dependent manner in Caenorhabditis elegans. Developmental Biology, 319(2), 267-272. https://dx.doi.org/10.1016/j.ydbio.2008.04.016

MLA:

Pacquelet, Anne, et al. "PAR-6 levels are regulated by NOS-3 in a CUL-2 dependent manner in Caenorhabditis elegans." Developmental Biology 319.2 (2008): 267-272.

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