A casein kinase 1 and PAR proteins regulate asymmetry of a PIP2 synthesis enzyme for asymmetric spindle positioning

Panbianco C, Weinkove D, Zanin E, Jones D, Divecha N, Gotta M, Ahringer J (2008)

Publication Status: Published

Publication Type: Journal article

Publication year: 2008

Journal

Developmental Cell Elsevier (Cell Press)

Publisher: CELL PRESS

Book Volume: 15

Pages Range: 198-208

Journal Issue: 2

DOI: 10.1016/j.devcel.2008.06.002

Abstract

Spindle positioning is an essential feature of asymmetric cell division. The conserved PAR proteins together with heterotrimeric G proteins control spindle positioning in animal cells, but how these are linked is not known. In C. elegans, PAR protein activity leads to asymmetric spindle placement through cortical asymmetry of G alpha regulators GPR-1/2. Here, we establish that the casein kinase 1 gamma CSNK-1 and a PIP2 synthesis enzyme (PPK-1) transduce PAR polarity to asymmetric G alpha regulation. PPK-1 is posteriorly enriched in the one-celled embryo through PAR and CSNK-1 activities. Loss of CSNK-1 1 causes uniformly high PPK-1 levels, high symmetric cortical levels of GPR-1/2 and LIN-5, and increased spindle pulling forces. In contrast, knockdown of ppk-1 leads to low GPR-1/2 levels and decreased spindle forces. Furthermore, loss of CSNK-1 leads to increased levels of PIP2. We propose that asymmetric generation of PIP2 by PPK-1 directs the posterior enrichment of GPR-1/2 and LIN-5, leading to posterior spindle displacement.

Authors with CRIS profile

Esther Zanin

Additional Organisation(s)

Professur für Experimentelle Molekulare Zelldynamik

Involved external institutions

Eidgenössische Technische Hochschule Zürich (ETHZ) / Swiss Federal Institute of Technology in Zurich CH Switzerland (CH) University of Geneva / Université de Genève (UNIGE) CH Switzerland (CH)

How to cite

APA:

Panbianco, C., Weinkove, D., Zanin, E., Jones, D., Divecha, N., Gotta, M., & Ahringer, J. (2008). A casein kinase 1 and PAR proteins regulate asymmetry of a PIP2 synthesis enzyme for asymmetric spindle positioning. Developmental Cell, 15(2), 198-208. https://dx.doi.org/10.1016/j.devcel.2008.06.002

MLA:

Panbianco, Costanza, et al. "A casein kinase 1 and PAR proteins regulate asymmetry of a PIP2 synthesis enzyme for asymmetric spindle positioning." Developmental Cell 15.2 (2008): 198-208.

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