MGluR7 is a presynaptic metabotropic glutamate receptor at ribbon synapses of inner hair cells

Klotz L, Enz R (2021)


Publication Type: Journal article

Publication year: 2021

Journal

Book Volume: 35

Article Number: e21855

Journal Issue: 11

DOI: 10.1096/fj.202100672R

Abstract

Glutamate is the most pivotal excitatory neurotransmitter in the central nervous system. Metabotropic glutamate receptors (mGluRs) dimerize and can couple to inhibitory intracellular signal cascades, thereby protecting glutamatergic neurons from excessive excitation and cell death. MGluR7 is correlated with age-related hearing deficits and noise-induced hearing loss; however its exact localization in the cochlea is unknown. Here, we analyzed the expression and localization of mGluR7a and mGluR7b in mouse cochlear wholemounts in detail, using confocal microscopy and 3D reconstructions. We observed a presynaptic localization of mGluR7a at inner hair cells (IHCs), close to the synaptic ribbon. To detect mGluR7b, newly generated antibodies were characterized and showed co-localization with mGluR7a at IHC ribbon synapses. Compared to the number of synaptic ribbons, the numbers of mGluR7a and mGluR7b puncta were reduced at higher frequencies (48 to 64 kHz) and in older animals (6 and 12 months). Previously, we reported a presynaptic localization of mGluR4 and mGluR8b at this synapse type. This enables the possibility for the formation of homo- and/or heterodimeric receptors composed of mGluR4, mGluR7a, mGluR7b and mGluR8b at IHC ribbon synapses. These receptor complexes might represent new molecular targets suited for pharmacological concepts to protect the cochlea against noxious stimuli and excitotoxicity.

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How to cite

APA:

Klotz, L., & Enz, R. (2021). MGluR7 is a presynaptic metabotropic glutamate receptor at ribbon synapses of inner hair cells. The FASEB Journal, 35(11). https://dx.doi.org/10.1096/fj.202100672R

MLA:

Klotz, Lisa, and Ralf Enz. "MGluR7 is a presynaptic metabotropic glutamate receptor at ribbon synapses of inner hair cells." The FASEB Journal 35.11 (2021).

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