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Microfluidics applications for high-throughput single cell sequencing

Zhou WM, Yan YY, Guo QR, Ji H, Wang H, Xu TT, Makabel B, Pilarsky C, He G, Yu XY, Zhang JY (2021)

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Publication Type: Journal article, Review article

Publication year: 2021

Journal

Book Volume: 19

Article Number: 312

Journal Issue: 1

DOI: 10.1186/s12951-021-01045-6

Abstract

The inherent heterogeneity of individual cells in cell populations plays significant roles in disease development and progression, which is critical for disease diagnosis and treatment. Substantial evidences show that the majority of traditional gene profiling methods mask the difference of individual cells. Single cell sequencing can provide data to characterize the inherent heterogeneity of individual cells, and reveal complex and rare cell populations. Different microfluidic technologies have emerged for single cell researches and become the frontiers and hot topics over the past decade. In this review article, we introduce the processes of single cell sequencing, and review the principles of microfluidics for single cell analysis. Also, we discuss the common high-throughput single cell sequencing technologies along with their advantages and disadvantages. Lastly, microfluidics applications in single cell sequencing technology for the diagnosis of cancers and immune system diseases are briefly illustrated. [Figure not available: see fulltext.]

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How to cite

APA:

Zhou, W.-M., Yan, Y.-Y., Guo, Q.-R., Ji, H., Wang, H., Xu, T.-T.,... Zhang, J.-Y. (2021). Microfluidics applications for high-throughput single cell sequencing. Journal of Nanobiotechnology, 19(1). https://dx.doi.org/10.1186/s12951-021-01045-6

MLA:

Zhou, Wen-Min, et al. "Microfluidics applications for high-throughput single cell sequencing." Journal of Nanobiotechnology 19.1 (2021).

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