Free Transplantation of a Tissue Engineered Bone Graft into an Irradiated, Critical-Size Femoral Defect in Rats

Rottensteiner-Brandl U, Bertram U, Lingens L, Köhn K, Distel L, Fey T, Körner C, Horch RE, Arkudas A (2021)

Publication Language: English

Publication Type: Journal article

Publication year: 2021

Journal

Cells MDPI

Book Volume: 10

Journal Issue: 9

DOI: 10.3390/cells10092256

Abstract

Healing of large bone defects remains a challenge in reconstructive surgery, especially with impaired healing potential due to severe trauma, infection or irradiation. In vivo studies are often performed in healthy animals, which might not accurately reflect the situation in clinical cases. In the present study, we successfully combined a critical-sized femoral defect model with an ionizing radiation protocol in rats. To support bone healing, tissue-engineered constructs were transferred into the defect after ectopic preossification and prevascularization. The combination of SiHA, MSCs and BMP-2 resulted in the significant ectopic formation of bone tissue, which can easily be transferred by means of our custom-made titanium chamber. Implanted osteogenic MSCs survived in vivo for a total of 18 weeks. The use of SiHA alone did not lead to bone formation after ectopic implantation. Analysis of gene expression showed early osteoblast differentiation and a hypoxic and inflammatory environment in implanted constructs. Irradiation led to impaired bone healing, decreased vascularization and lower short-term survival of implanted cells. We conclude that our model is highly valuable for the investigation of bone healing and tissue engineering in pre-damaged tissue and that healing of bone defects can be substantially supported by combining SiHA, MSCs and BMP-2.

Authors with CRIS profile

Ulrike Rottensteiner-Brandl Department of Plastic and Hand Surgery Ulf Bertram Department of Plastic and Hand Surgery Lara Lingens Department of Plastic and Hand Surgery Katrin Köhn Department of Plastic and Hand Surgery Luitpold Distel Department of Radiation Oncology Tobias Fey Lehrstuhl für Glas und Keramik Carolin Körner Lehrstuhl für Werkstoffwissenschaften (Werkstoffkunde und Technologie der Metalle) Raymund Horch Professur für Plastische Chirurgie und Handchirurgie Andreas Arkudas Medizinische Fakultät

How to cite

APA:

Rottensteiner-Brandl, U., Bertram, U., Lingens, L., Köhn, K., Distel, L., Fey, T.,... Arkudas, A. (2021). Free Transplantation of a Tissue Engineered Bone Graft into an Irradiated, Critical-Size Femoral Defect in Rats. Cells, 10(9). https://dx.doi.org/10.3390/cells10092256

MLA:

Rottensteiner-Brandl, Ulrike, et al. "Free Transplantation of a Tissue Engineered Bone Graft into an Irradiated, Critical-Size Femoral Defect in Rats." Cells 10.9 (2021).

BibTeX: Download