Six-Color Confocal Immunofluorescence Microscopy with 4-Laser Lines

Heger L, Lühr JJ, Amon L, Smith AS, Eissing N, Dudziak D (2021)

Publication Type: Book chapter / Article in edited volumes

Publication year: 2021

Journal

Publisher: Humana Press Inc.

Edited Volumes: Methods in Molecular Biology

Book Volume: 2350

Pages Range: 21-30

DOI: 10.1007/978-1-0716-1593-5_2

Abstract

Confocal immunofluorescence microscopy is an advanced imaging technique routinely applied in the laboratory and clinics. Histological analyses are performed from tissue material. In general, a single fluorochrome per laser is employed, limiting simultaneous analysis to four antigens in one staining with a conventional 4-laser line microscope. Here, we describe a protocol for combining fluorochromes with the same excitation but different emission properties that allows for the analysis of six different antigens in confocal immunofluorescence microscopy with a conventional 4-laser line microscope. The proposed multiplexed method permits the identification and characterization of complex cell populations in rare tissue material.

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Additional Organisation(s)

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Involved external institutions

Max-Planck-Institut für die Physik des Lichts (MPL) / Max Planck Institute for the Science of Light Germany (DE)

How to cite

APA:

Heger, L., Lühr, J.J., Amon, L., Smith, A.-S., Eissing, N., & Dudziak, D. (2021). Six-Color Confocal Immunofluorescence Microscopy with 4-Laser Lines. In Methods in Molecular Biology. (pp. 21-30). Humana Press Inc..

MLA:

Heger, Lukas, et al. "Six-Color Confocal Immunofluorescence Microscopy with 4-Laser Lines." Methods in Molecular Biology. Humana Press Inc., 2021. 21-30.

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