Distinct endocytosis and immune activation of poly lactic-co-glycolic acid nanoparticles prepared by single- and double-emulsion evaporation

Spiecker E, Hackstein H, Wu M, Scheuer K, Brox R, Apeleo Zubiri B, Pusch L, Jandt KD, Yokosawa T, Fischer D (2021)

Publication Type: Other publication type

Publication year: 2021

Journal

Nanomedicine Future Medicine

Book Volume: 16

Pages Range: 2075-2094

Journal Issue: 23

DOI: 10.2217/nnm-2021-0022

Abstract

Background: Poly lactic-co-glycolic acid (PLGA) nanoparticles can be prepared by emulsion-solvent-evaporation from o/w and w(1)/o/w(2) emulsions. Aims: To elaborate similarities and differences regarding mechanical, morphological and physicochemical properties, as well as endocytosis and dose-dependent immune responses by primary human leukocytes between nanoparticles prepared by these two methods. Methods: Fluorescently labeled as well as TLR-agonist (R848)-loaded PLGA nanoparticles were prepared via both single- and double-emulsion solvent evaporation. Results: Particles prepared by both methods were similar in chemical composition and surface charge but exhibited slight differences in size and morphology. Pronounced differences were found for loading, dissolution and mechanical properties. The particles were differently endocytosed by monocytes and induced qualitatively and quantitatively different immune responses. Conclusions: Variations in nanoparticle preparation can affect particle-derived immunological characteristics.

Authors with CRIS profile

Erdmann Spiecker Lehrstuhl für Werkstoffwissenschaften (Mikro- und Nanostrukturforschung) Holger Hackstein Professur für Transfusionsmedizin und Cell-Engineering Mingjian Wu Lehrstuhl für Werkstoffwissenschaften (Mikro- und Nanostrukturforschung) Regine Brox Department of Transfusion Medicine and Haemostaseology Benjamin Apeleo Zubiri Lehrstuhl für Werkstoffwissenschaften (Mikro- und Nanostrukturforschung) Tadahiro Yokosawa Lehrstuhl für Werkstoffwissenschaften (Mikro- und Nanostrukturforschung) Dagmar Fischer Lehrstuhl für Pharmazeutische Technologie und Biopharmazie

Additional Organisation(s)

Interdisziplinäres Zentrum, Center for Nanoanalysis and Electron Microscopy (CENEM)

Involved external institutions

Friedrich-Schiller-Universität Jena DE Germany (DE)

How to cite

APA:

Spiecker, E., Hackstein, H., Wu, M., Scheuer, K., Brox, R., Apeleo Zubiri, B.,... Fischer, D. (2021). Distinct endocytosis and immune activation of poly lactic-co-glycolic acid nanoparticles prepared by single- and double-emulsion evaporation.

MLA:

Spiecker, Erdmann, et al. Distinct endocytosis and immune activation of poly lactic-co-glycolic acid nanoparticles prepared by single- and double-emulsion evaporation. 2021.

BibTeX: Download