Citrate-coated superparamagnetic iron oxide nanoparticles enable a stable non-spilling loading of t cells and their magnetic accumulation

Boosz P, Pfister F, Stein R, Friedrich B, Fester L, Band J, Mühlberger M, Schreiber E, Lyer S, Dudziak D, Alexiou C, Janko C (2021)

Publication Type: Journal article

Publication year: 2021

Journal

Cancers MDPI

Book Volume: 13

Article Number: 4143

Journal Issue: 16

DOI: 10.3390/cancers13164143

Abstract

T cell infiltration into a tumor is associated with a good clinical prognosis of the patient and adoptive T cell therapy can increase anti-tumor immune responses. However, immune cells are often excluded from tumor infiltration and can lack activation due to the immune-suppressive tumor microenvironment. To make T cells controllable by external forces, we loaded primary human CD3+ T cells with citrate-coated superparamagnetic iron oxide nanoparticles (SPIONs). Since the efficacy of magnetic targeting depends on the amount of SPION loading, we investigated how experimental conditions influence nanoparticle uptake and viability of cells. We found that loading in the presence of serum improved both the colloidal stability of SPIONs and viability of T cells, whereas stimulation with CD3/CD28/CD2 and IL-2 did not influence nanoparticle uptake. Further-more, SPION loading did not impair cytokine secretion after polyclonal stimulation. We finally achieved 1.4 pg iron loading per cell, which was both located intracellularly in vesicles and bound to the plasma membrane. Importantly, nanoparticles did not spill over to non-loaded cells. Since SPION-loading enabled efficient magnetic accumulation of T cells in vitro under dynamic condi-tions, we conclude that this might be a good starting point for the investigation of in vivo delivery of immune cells.

Authors with CRIS profile

Philipp Boosz Professur für Nanomedizin Felix Pfister Department of Otorhinolaryngology – Head and Neck Surgery René Stein Department of Otorhinolaryngology – Head and Neck Surgery Bernhard Friedrich Department of Otorhinolaryngology – Head and Neck Surgery Lars Fester Professur für Neuromorphologie Marina Mühlberger Department of Otorhinolaryngology – Head and Neck Surgery Stefan Lyer Department of Otorhinolaryngology – Head and Neck Surgery Diana Dudziak Professur für die Biologie Dendritischer Zellen Christoph Alexiou Professur für Nanomedizin Christina Janko Department of Otorhinolaryngology – Head and Neck Surgery

How to cite

APA:

Boosz, P., Pfister, F., Stein, R., Friedrich, B., Fester, L., Band, J.,... Janko, C. (2021). Citrate-coated superparamagnetic iron oxide nanoparticles enable a stable non-spilling loading of t cells and their magnetic accumulation. Cancers, 13(16). https://dx.doi.org/10.3390/cancers13164143

MLA:

Boosz, Philipp, et al. "Citrate-coated superparamagnetic iron oxide nanoparticles enable a stable non-spilling loading of t cells and their magnetic accumulation." Cancers 13.16 (2021).

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