Homodimerization of a proximal region within the C-terminus of the orphan G-protein coupled receptor GPR179

Bachert W, Klotz L, Sticht H, Enz R (2021)

Publication Type: Journal article

Publication year: 2021

Journal

Book Volume: 149

Article Number: 105150

DOI: 10.1016/j.neuint.2021.105150

Abstract

G-protein coupled receptors exhibit numerous biological functions. The orphan G-protein coupled receptor GPR179 is a central component of a 1 Megadalton large signalling complex in the ON-pathway of the mammalian retina that assembles multiple proteins, including the metabotropic glutamate receptor mGluR6. Dimer formation is a hallmark of G-protein coupled receptors and some use intracellular C-termini for dimerization. Here we tested the dimerization properties of the intracellular C-terminal domains of mGluR6 and GPR179. While the C-termini of GPR179 and mGluR6 did not interact, we detected a robust homodimerization of a proximal region in the GPR179 C-terminus. Mapping studies defined a linear stretch of 64 amino acids as dimerization region. Bioinformatic analysis indicated that this dimerization region might adopt an α-helical structure that is predicted to dimerize by forming a coiled-coil. Based on these data, we speculate that homodimerization of GPR179 might contribute to the formation of large signalling complexes in the mammalian retina.

Authors with CRIS profile

How to cite

APA:

Bachert, W., Klotz, L., Sticht, H., & Enz, R. (2021). Homodimerization of a proximal region within the C-terminus of the orphan G-protein coupled receptor GPR179. Neurochemistry International, 149. https://dx.doi.org/10.1016/j.neuint.2021.105150

MLA:

Bachert, Wolfrat, et al. "Homodimerization of a proximal region within the C-terminus of the orphan G-protein coupled receptor GPR179." Neurochemistry International 149 (2021).

BibTeX: Download