Cell types and synapses expressing the snare complex regulating proteins complexin 1 and complexin 2 in mammalian retina
Lux U, Ehrenberg J, Joachimsthaler A, Atorf J, Pircher B, Reim K, Kremers J, Gießl A, Brandstätter JH (2021)
Publication Type: Journal article
Publication year: 2021
Journal
Book Volume: 22
Article Number: 8131
Journal Issue: 15
DOI: 10.3390/ijms22158131
Abstract
Complexins (Cplxs) 1 to 4 are components of the presynaptic compartment of chemical synapses where they regulate important steps in synaptic vesicle exocytosis. In the retina, all four Cplxs are present, and while we know a lot about Cplxs 3 and 4, little is known about Cplxs 1 and 2. Here, we performed in situ hybridization experiments and bioinformatics and exploited Cplx 1 and Cplx 2 single-knockout mice combined with immunocytochemistry and light microscopy to characterize in detail the cell type and synapse-specific distribution of Cplx 1 and Cplx 2. We found that Cplx 2 and not Cplx 1 is the main isoform expressed in normal and displaced amacrine cells and ganglion cells in mouse retinae and that amacrine cells seem to operate with a single Cplx isoform at their conventional chemical synapses. Surprising was the finding that retinal function, determined with electroretinographic recordings, was altered in Cplx 1 but not Cplx 2 single-knockout mice. In summary, the results provide an important basis for future studies on the function of Cplxs 1 and 2 in the processing of visual signals in the mammalian retina.
Authors with CRIS profile
Uwe Lux
Lehrstuhl für Tierphysiologie
Anneka Joachimsthaler
Department of Ophthalmology
Jenny Atorf
Department of Ophthalmology
Bianca Pircher
Professur für Neurobiologie
Jan Kremers
Professur für Experimentelle Augenheilkunde
Andreas Gießl
Department of Ophthalmology
Johann Helmut Brandstätter
Lehrstuhl für Tierphysiologie
Involved external institutions
Germany (DE)How to cite
APA:
Lux, U., Ehrenberg, J., Joachimsthaler, A., Atorf, J., Pircher, B., Reim, K.,... Brandstätter, J.H. (2021). Cell types and synapses expressing the snare complex regulating proteins complexin 1 and complexin 2 in mammalian retina. International Journal of Molecular Sciences, 22(15). https://dx.doi.org/10.3390/ijms22158131
MLA:
Lux, Uwe, et al. "Cell types and synapses expressing the snare complex regulating proteins complexin 1 and complexin 2 in mammalian retina." International Journal of Molecular Sciences 22.15 (2021).
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