Monomethine cyanine probes for visualization of cellular RNA by fluorescence microscopy
Aristova D, Kosach V, Chernii S, Slominsky Y, Balanda A, Filonenko V, Yarmoluk S, Rotaru A, Özkan H, Mokhir A, Kovalska VB (2021)
Publication Type: Journal article
Publication year: 2021
Journal
Book Volume: 9
Journal Issue: 4
Abstract
We have studied spectral-luminescent properties of the monomethine cyanine dyes both in their free states and in the presence of either double-stranded deoxyribonucleic acids (dsDNAs) or single-stranded ribonucleic acids (RNAs). The dyes possess low fluorescence intensity in an unbound state, which is increased up to 479 times in the presence of the nucleic acids. In the presence of RNAs, the fluorescence intensity increase was stronger than that observed in the presence of dsDNA. Next, we have performed staining of live and fixed cells by all prepared dyes. The dyes proved to be cell and nuclear membrane permeant. They are photostable and brightly stain RNA-containing organelles in both live and fixed cells. The colocalization confirmed the specific nucleoli staining with anti-Ki-67 antibodies. The RNA digestion experiment has confirmed the selectivity of the dyes toward intracellular RNA. Based on the obtained results, we can conclude that the investigated monomethine cyanine dyes are useful fluorescent probes for the visualization of intracellular RNA and RNA-containing organelles such as nucleoli by using fluorescence microscopy.
Authors with CRIS profile
Involved external institutions
Institute of Organic Chemistry of the NAS of Ukraine / Iнститут органiчної хiмiї НАН України
Ukraine (UA)
Institute of Molecular Biology and Genetics of NASU / Інститут молекулярної біології і генетики
Ukraine (UA)
Romanian Academy / Academia Română
Romania (RO)
National Academy of Sciences of Ukraine (NAN) / Національна академія наук України
Ukraine (UA)
Ukraine (UA)
Institute of Molecular Biology and Genetics of NASU / Інститут молекулярної біології і генетики
Ukraine (UA)
Romanian Academy / Academia Română
Romania (RO)
National Academy of Sciences of Ukraine (NAN) / Національна академія наук України
Ukraine (UA)
How to cite
APA:
Aristova, D., Kosach, V., Chernii, S., Slominsky, Y., Balanda, A., Filonenko, V.,... Kovalska, V.B. (2021). Monomethine cyanine probes for visualization of cellular RNA by fluorescence microscopy. Methods and Applications in Fluorescence, 9(4). https://dx.doi.org/10.1088/2050-6120/ac10ad
MLA:
Aristova, Daria, et al. "Monomethine cyanine probes for visualization of cellular RNA by fluorescence microscopy." Methods and Applications in Fluorescence 9.4 (2021).
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