Biomimetic calcium phosphate coatings for bioactivation of titanium implant surfaces: Methodological approach and in vitro evaluation of biocompatibility

Kreller T, Sahm F, Bader R, Boccaccini AR, Jonitz-Heincke A, Detsch R (2021)

Publication Type: Journal article

Publication year: 2021

Journal

Materials MDPI

Book Volume: 14

Article Number: 3516

Journal Issue: 13

DOI: 10.3390/ma14133516

Abstract

Ti6Al4V as a common implant material features good mechanical properties and corrosion resistance. However, untreated, it lacks bioactivity. In contrast, coatings with calcium phosphates (CaP) were shown to improve cell–material interactions in bone tissue engineering. Therefore, this work aimed to investigate how to tailor biomimetic CaP coatings on Ti6Al4V substrates using modified biomimetic calcium phosphate (BCP) coating solutions. Furthermore, the impact of substrate immersion in a 1 M alkaline CaCl2 solution (pH = 10) on subsequent CaP coating formation was examined. CaP coatings were characterized via scanning electron microscopy, x-ray diffraction, energy-dispersive x-ray spectroscopy, and laser-scanning microscope. Biocompatibility of coatings was carried out with primary human osteoblasts analyzing cell morphology, proliferation, collagen type 1, and interleukin 6 and 8 release. Results indicate a successful formation of low crystalline hydroxyapatite (HA) on top of every sample after immersion in each BCP coating solution after 14 days. Furthermore, HA coating promoted cell proliferation and reduced the concentration of interleukins compared to the uncoated surface, assuming increased biocompatibility.

Authors with CRIS profile

Thomas Kreller Lehrstuhl für Werkstoffwissenschaften (Biomaterialien) Aldo Boccaccini Lehrstuhl für Werkstoffwissenschaften (Biomaterialien) Rainer Detsch Lehrstuhl für Werkstoffwissenschaften (Biomaterialien)

Involved external institutions

Universitätsmedizin Rostock DE Germany (DE)

How to cite

APA:

Kreller, T., Sahm, F., Bader, R., Boccaccini, A.R., Jonitz-Heincke, A., & Detsch, R. (2021). Biomimetic calcium phosphate coatings for bioactivation of titanium implant surfaces: Methodological approach and in vitro evaluation of biocompatibility. Materials, 14(13). https://dx.doi.org/10.3390/ma14133516

MLA:

Kreller, Thomas, et al. "Biomimetic calcium phosphate coatings for bioactivation of titanium implant surfaces: Methodological approach and in vitro evaluation of biocompatibility." Materials 14.13 (2021).

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