The phragmoplast-orienting kinesin-12 class proteins translate the positional information of the preprophase band to establish the cortical division zone in Arabidopsis thaliana

Lipka E, Gadeyne A, Stoeckle D, Zimmermann S, De Jaeger G, Ehrhardt DW, Kirik V, Van Damme D, Müller S (2014)

Publication Type: Journal article

Publication year: 2014

Journal

The Plant Cell American Society of Plant Biologists

Book Volume: 26

Pages Range: 2617-2632

Journal Issue: 6

DOI: 10.1105/tpc.114.124933

Abstract

The preprophase band (PPB) is a faithful but transient predictor of the division plane in somatic cell divisions. Throughout mitosis the PPBs positional information is preserved by factors that continuously mark the division plane at the cell cortex, the cortical division zone, by their distinct spatio-temporal localization patterns. However, the mechanism maintaining these identity factors at the plasma membrane after PPB disassembly remains obscure. The pair of kinesin-12 class proteins PHRAGMOPLAST ORIENTING KINESIN1 (POK1) and POK2 are key players in division plane maintenance. Here, we show that POK1 is continuously present at the cell cortex, providing a spatial reference for the site formerly occupied by the PPB. Fluorescence recovery after photobleaching analysis combined with microtubule destabilization revealed dynamic microtubule-dependent recruitment of POK1 to the PPB during prophase, while POK1 retention at the cortical division zone in the absence of cortical microtubules appeared static. POK function is strictly required to maintain the division plane identity factor TANGLED (TAN) after PPB disassembly, although POK1 and TAN recruitment to the PPB occur independently during prophase. Together, our data suggest that POKs represent fundamental early anchoring components of the cortical division zone, translating and preserving the positional information of the PPB by maintaining downstream identity markers. © 2014 American Society of Plant Biologists. All rights reserved.

Authors with CRIS profile

Sabine Müller

Involved external institutions

Eberhard Karls Universität Tübingen DE Germany (DE) Flanders Institute for Biotechnology / Vlaams Instituut voor Biotechnologie (VIB) BE Belgium (BE) Carnegie Institution for Science US United States (USA) (US) Illinois State University (ISU) US United States (USA) (US)

How to cite

APA:

Lipka, E., Gadeyne, A., Stoeckle, D., Zimmermann, S., De Jaeger, G., Ehrhardt, D.W.,... Müller, S. (2014). The phragmoplast-orienting kinesin-12 class proteins translate the positional information of the preprophase band to establish the cortical division zone in Arabidopsis thaliana. The Plant Cell, 26(6), 2617-2632. https://dx.doi.org/10.1105/tpc.114.124933

MLA:

Lipka, Elisabeth, et al. "The phragmoplast-orienting kinesin-12 class proteins translate the positional information of the preprophase band to establish the cortical division zone in Arabidopsis thaliana." The Plant Cell 26.6 (2014): 2617-2632.

BibTeX: Download