Engineering strategies for oral therapeutic enzymes to enhance their stability and activity
Lapuhs P, Fuhrmann G (2019)
Publication Type: Book chapter / Article in edited volumes
Publication year: 2019
Journal
Publisher: Springer New York LLC
Edited Volumes: Advances in Experimental Medicine and Biology
Series: Advances in Experimental Medicine and Biology
Book Volume: 1148
Pages Range: 151-172
DOI: 10.1007/978-981-13-7709-9_8
Abstract
Oral application of therapeutic enzymes is a promising and non-invasive administration that improves patient compliance. However, the gastrointestinal tract poses several challenges to the oral delivery of proteins, including harsh pH conditions and digestive proteases. A promising way to stabilise enzymes during their gastrointestinal route is by modification with polymers that can provide both steric shielding and selective interaction in different digestive compartments. We give an overview of modification technologies for oral enzymes ranging from functionalisation of native proteins, to site-specific mutation and protein-polymer engineering. We specifically focus on enzymes that are active directly in the gastrointestinal lumen and not systemically absorbed. In addition, we discuss examples of microparticle and nanoparticle encapsulated enzymes for improved oral delivery. The modification of orally administered enzymes offers a broad chemical variability and may be a promising tool for enhancing their gastrointestinal stability.
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Germany (DE)How to cite
APA:
Lapuhs, P., & Fuhrmann, G. (2019). Engineering strategies for oral therapeutic enzymes to enhance their stability and activity. In Advances in Experimental Medicine and Biology. (pp. 151-172). Springer New York LLC.
MLA:
Lapuhs, Philipp, and Gregor Fuhrmann. "Engineering strategies for oral therapeutic enzymes to enhance their stability and activity." Advances in Experimental Medicine and Biology. Springer New York LLC, 2019. 151-172.
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