Cold atmospheric plasma promotes the immunoreactivity of granulocytes in vitro

Kupke LS, Arndt S, Lenzer S, Metz S, Unger P, Zimmermann JL, Boßerhoff AK, Gruber M, Karrer S (2021)

Publication Type: Journal article

Publication year: 2021

Journal

Biomolecules MDPI

Book Volume: 11

Article Number: 902

Journal Issue: 6

DOI: 10.3390/biom11060902

Abstract

Cold atmospheric plasma (CAP) reduces bacteria and interacts with tissues and cells, thus improving wound healing. The CAP-related induction of neutrophils was recently described in stained sections of wound tissue in mice. Consequently, this study aimed to examine the functionality of human polymorphonuclear cells (PMN)/granulocytes through either a plasma-treated solution (PTS) or the direct CAP treatment with different plasma modes and treatment durations. PTS analysis yielded mode-dependent differences in the production of reactive oxygen species (ROS) and reactive nitrogen species (RNS) after CAP treatment. Live-cell imaging did not show any chemo-attractive or NETosis-inducing effect on PMNs treated with PTS. The time to maximum ROS production (TmaxROS) in PMNs was reduced by PTS and direct CAP treatment. PMNs directly treated with CAP showed an altered cell migration dependent on the treatment duration as well as decreased TmaxROS without inducing apoptosis. Additionally, flow cytometry showed enhanced integrin and selectin expression, as a marker of activation, on PMN surfaces. In conclusion, the modification of PMN immunoreactivity may be a main supporting mechanism for CAP-induced improvement in wound healing.

Authors with CRIS profile

Anja Katrin Boßerhoff Lehrstuhl für Biochemie und Molekulare Medizin

Involved external institutions

Universitätsklinikum Regensburg DE Germany (DE) terraplasma GmbH DE Germany (DE)

How to cite

APA:

Kupke, L.S., Arndt, S., Lenzer, S., Metz, S., Unger, P., Zimmermann, J.L.,... Karrer, S. (2021). Cold atmospheric plasma promotes the immunoreactivity of granulocytes in vitro. Biomolecules, 11(6). https://dx.doi.org/10.3390/biom11060902

MLA:

Kupke, Laura S., et al. "Cold atmospheric plasma promotes the immunoreactivity of granulocytes in vitro." Biomolecules 11.6 (2021).

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