Isolation of sesquiterpenoids from Matricaria chamomilla by means of solvent assisted flavor evaporation and centrifugal partition chromatography
Slavik B, Roehrer S, Loos H, Minceva M, Büttner A (2021)
Publication Type: Journal article
Publication year: 2021
Journal
DOI: 10.1007/s00216-021-03400-w
Abstract
The (semi)volatile fraction of Matricaria chamomilla L., an annual herbal plant from the family of Asteraceae, contains high quantities of sesquiterpenes and sesquiterpenoids. A method was developed to achieve isolation and separation of these compounds, using a combination of solvent assisted flavor evaporation (SAFE) and solid support-free liquid-liquid chromatography. The biphasic liquid solvent system n-heptane/ethyl acetate/methanol/water, 5/2/5/2 v/v/v/v (Arizona S) was elaborated as a suitable solvent system for the simultaneous separation of the target compounds. The lab-scale liquid-liquid chromatography separation performed in a countercurrent chromatography (CCC) column was successfully transferred to a semi-preparative centrifugal partition chromatography (CPC) column, which enabled the isolation of artemisia ketone, artemisia alcohol, α-bisabolone oxide A, and (E)-en-yn-dicycloether. α-Bisabolol oxide A and (Z)-en-yn-dicycloether co-eluted, but were successfully separated by subsequent size-exclusion chromatography (SEC). Similarly, spathulenol and α-bisabolol oxide B were obtained as a mixture, and were separated by means of column chromatography using silica gel as stationary phase. The isolated compounds were characterized by means of nuclear magnetic resonance spectroscopy (NMR) and gas chromatography–mass spectrometry (GC-MS). Graphical abstract: [Figure not available: see fulltext.]
Authors with CRIS profile
Benedikt Slavik
Lehrstuhl für Aroma- und Geruchsforschung
Helene Loos
Lehrstuhl für Aroma- und Geruchsforschung
Andrea Büttner
Lehrstuhl für Aroma- und Geruchsforschung
Additional Organisation(s)
Involved external institutions
Germany (DE)How to cite
APA:
Slavik, B., Roehrer, S., Loos, H., Minceva, M., & Büttner, A. (2021). Isolation of sesquiterpenoids from Matricaria chamomilla by means of solvent assisted flavor evaporation and centrifugal partition chromatography. Analytical and Bioanalytical Chemistry. https://doi.org/10.1007/s00216-021-03400-w
MLA:
Slavik, Benedikt, et al. "Isolation of sesquiterpenoids from Matricaria chamomilla by means of solvent assisted flavor evaporation and centrifugal partition chromatography." Analytical and Bioanalytical Chemistry (2021).
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