Cortactin is required for efficient fak, src and abl tyrosine kinase activation and phosphorylation of helicobacter pylori caga
Knorr J, Sharafutdinov I, Fiedler F, Soltan Esmaeili D, Rohde M, Rottner K, Backert S, Tegtmeyer N (2021)
Publication Type: Journal article
Publication year: 2021
Journal
Book Volume: 22
Article Number: 6045
Journal Issue: 11
DOI: 10.3390/ijms22116045
Abstract
Cortactin is a well‐known regulatory protein of the host actin cytoskeleton and represents an attractive target of microbial pathogens like Helicobacter pylori. H. pylori manipulates cortactin’s phosphorylation status by type‐IV secretion‐dependent injection of its virulence protein CagA. Multiple host tyrosine kinases, like FAK, Src, and Abl, are activated during infection, but the pathway(s) involved is (are) not yet fully established. Among them, Src and Abl target CagA and stimulate tyrosine phosphorylation of the latter at its EPIYA‐motifs. To investigate the role of cortactin in more detail, we generated a CRISPR/Cas9 knockout of cortactin in AGS gastric epithelial cells. Surprisingly, we found that FAK, Src, and Abl kinase activities were dramatically downregulated associated with widely diminished CagA phosphorylation in cortactin knockout cells compared to the parental control. Together, we report here a yet unrecognized cortactin‐dependent signaling pathway involving FAK, Src, and Abl activation, and controlling efficient phosphorylation of injected CagA during infection. Thus, the cortactin status could serve as a potential new biomarker of gastric cancer development.
Authors with CRIS profile
Jakob Knorr
Lehrstuhl für Mikrobiologie
Irshad Sharafutdinov
Lehrstuhl für Mikrobiologie
Florian Fiedler
Lehrstuhl für Mikrobiologie
Delara Soltan Esmaeili
Lehrstuhl für Mikrobiologie
Steffen Backert
Lehrstuhl für Mikrobiologie
Nicole Tegtmeyer-Backert
Lehrstuhl für Mikrobiologie
Involved external institutions
Germany (DE)How to cite
APA:
Knorr, J., Sharafutdinov, I., Fiedler, F., Soltan Esmaeili, D., Rohde, M., Rottner, K.,... Tegtmeyer, N. (2021). Cortactin is required for efficient fak, src and abl tyrosine kinase activation and phosphorylation of helicobacter pylori caga. International Journal of Molecular Sciences, 22(11). https://dx.doi.org/10.3390/ijms22116045
MLA:
Knorr, Jakob, et al. "Cortactin is required for efficient fak, src and abl tyrosine kinase activation and phosphorylation of helicobacter pylori caga." International Journal of Molecular Sciences 22.11 (2021).
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