The UBXN-2/p37/p47 adaptors of CDC-48/p97 regulate mitosis by limiting the centrosomal recruitment of Aurora A
Kress E, Schwager F, Holtackers R, Seiler J, Prodon F, Zanin E, Eiteneuer A, Toya M, Sugimoto A, Meyer H, Meraldi P, Gotta M (2013)
Publication Type: Journal article
Publication year: 2013
Journal
Book Volume: 201
Pages Range: 559-575
Journal Issue: 4
Abstract
Coordination of cell cycle events in space and time is crucial to achieve a successful cell division. Here, we demonstrate that UBXN-2, a substrate adaptor of the AAA ATPase Cdc48/p97, is required to coordinate centrosome maturation timing with mitosis. In UBXN-2-depleted Caenorhabditis elegans embryos, centrosomes recruited more AIR-1 (Aurora A), matured precociously, and alignment of the mitotic spindle with the axis of polarity was impaired. UBXN-2 and CDC-48 coimmunoprecipitated with AIR-1 and the spindle alignment defect was partially rescued by co-depleting AIR-1, indicating that UBXN-2 controls these processes via AIR-1. Similarly, depletion in human cells of the UBXN-2 orthologues p37/p47 resulted in an accumulation of Aurora A at centrosomes and a delay in centrosome separation. The latter defect was also rescued by inhibiting Aurora A. We therefore postulate that the role of this adaptor in cell cycle regulation is conserved. © 2013 Kress et al.
Authors with CRIS profile
Additional Organisation(s)
Involved external institutions
University of Geneva / Université de Genève (UNIGE)
Switzerland (CH)
Eidgenössische Technische Hochschule Zürich (ETHZ) / Swiss Federal Institute of Technology in Zurich
Switzerland (CH)
Universität Duisburg-Essen (UDE)
Germany (DE)
University of California, San Diego
United States (USA) (US)
Riken Institute of Physical and Chemical Research / 理研
Japan (JP)
Tohoku University
Japan (JP)
Switzerland (CH)
Eidgenössische Technische Hochschule Zürich (ETHZ) / Swiss Federal Institute of Technology in Zurich
Switzerland (CH)
Universität Duisburg-Essen (UDE)
Germany (DE)
University of California, San Diego
United States (USA) (US)
Riken Institute of Physical and Chemical Research / 理研
Japan (JP)
Tohoku University
Japan (JP)
How to cite
APA:
Kress, E., Schwager, F., Holtackers, R., Seiler, J., Prodon, F., Zanin, E.,... Gotta, M. (2013). The UBXN-2/p37/p47 adaptors of CDC-48/p97 regulate mitosis by limiting the centrosomal recruitment of Aurora A. The Journal of Cell Biology, 201(4), 559-575. https://dx.doi.org/10.1083/jcb.201209107
MLA:
Kress, Elsa, et al. "The UBXN-2/p37/p47 adaptors of CDC-48/p97 regulate mitosis by limiting the centrosomal recruitment of Aurora A." The Journal of Cell Biology 201.4 (2013): 559-575.
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