A conserved RhoGAP limits M phase contractility and coordinates with microtubule asters to confine RhoA during Cytokinesis
Zanin E, Desai A, Poser I, Toyoda Y, Andree C, Moebius C, Bickle M, Conradt B, Piekny A, Oegema K (2013)
Publication Type: Journal article
Publication year: 2013
Journal
Book Volume: 26
Pages Range: 496-510
Journal Issue: 5
DOI: 10.1016/j.devcel.2013.08.005
Abstract
During animal cell cytokinesis, the spindle directs contractile ring assembly by activating RhoA in a narrow equatorial zone. Rapid GTPase activating protein (GAP)-mediated inactivation (RhoA flux) is proposed to limit RhoA zone dimensions. Testing the significance of RhoA flux has been hampered by the fact that the GAP targeting RhoA is not known. Here, we identify M phase GAP (MP-GAP) as the primary GAP targeting RhoA during mitosis and cytokinesis. MP-GAP inhibition caused excessive RhoA activation in M phase, leading to the uncontrolled formation of large cortical protrusions and late cytokinesis failure. RhoA zone width was broadened by attenuation of the centrosomal asters but was not affected by MP-GAP inhibition alone. Simultaneous aster attenuation and MP-GAP inhibition led to RhoA accumulation around the entire cell periphery. These results identify the major GAP restraining RhoA during cell division and delineate the relative contributions of RhoA flux and centrosomal asters in controlling RhoA zone dimensions. © 2013 Elsevier Inc.
Authors with CRIS profile
Additional Organisation(s)
Involved external institutions
Ludwig-Maximilians-Universität (LMU)
Germany (DE)
University of California, San Diego
United States (USA) (US)
Max-Planck-Institut für molekulare Zellbiologie und Genetik / Max Planck Institute of Molecular Cell Biology and Genetics (MPI-CBG)
Germany (DE)
Concordia University
Canada (CA)
Germany (DE)
University of California, San Diego
United States (USA) (US)
Max-Planck-Institut für molekulare Zellbiologie und Genetik / Max Planck Institute of Molecular Cell Biology and Genetics (MPI-CBG)
Germany (DE)
Concordia University
Canada (CA)
How to cite
APA:
Zanin, E., Desai, A., Poser, I., Toyoda, Y., Andree, C., Moebius, C.,... Oegema, K. (2013). A conserved RhoGAP limits M phase contractility and coordinates with microtubule asters to confine RhoA during Cytokinesis. Developmental Cell, 26(5), 496-510. https://dx.doi.org/10.1016/j.devcel.2013.08.005
MLA:
Zanin, Esther, et al. "A conserved RhoGAP limits M phase contractility and coordinates with microtubule asters to confine RhoA during Cytokinesis." Developmental Cell 26.5 (2013): 496-510.
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